![Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) APC.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-HIP1-Flow-Cytometry-NB500-511-img0004.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) APC.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | Flow, IHC, CyTOF-ready |
| Clone | HIP1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone HIP1 was used by HLDA to establish CD designation. |
| Immunogen | Peripheral blood mononuclear cells of a patient suffering with CLL. |
| Specificity | The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GP1BA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Functional Application: The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. This antibody is CyTOF ready. |
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| Theoretical MW | 145 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human platelets with anti-CD42b (HIP1) PE.](http://images.novusbio.com/fullsize/CD42b-GPIb-alpha-Antibody-HIP1-Flow-Cytometry-NB500-511-img0001.jpg "Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human platelets with anti-CD42b (HIP1) PE.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")