Clone HIP1 was used by HLDA to establish CD designation.
Immunogen
Peripheral blood mononuclear cells of a patient suffering with CLL.
Specificity
The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GP1BA
Purity
Protein A purified
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Functional Application: The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. This antibody is CyTOF ready.
Theoretical MW
145 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB500-511 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 31467123).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified
Alternate Names for CD42b/GPIb alpha Antibody (HIP1) - BSA Free
Antigen CD42b-alpha
BP1BA
BSS
CD42b antigen
CD42b
CD42b-alpha
glycoprotein Ib (platelet), alpha polypeptide
Glycoprotein Ibalpha
GP1B
GP1BA
GPIb alpha
GP-Ib alpha
GPIbA
GPIb-alpha
MGC34595
platelet glycoprotein Ib alpha chain
platelet membrane glycoprotein 1b-alpha subunit
Background
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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FAQs for CD42b/GPIb alpha Antibody (NB500-511). (Showing 1 - 1 of 1 FAQs).
I want to buy the anti- CD42b/GPIbA monclonal antibody, which is used to inject into the mouse to make the animal model of thrombopenia. Can the anti- CD42b/GPIbA antibody(NB500-511) be used in this experiment?
NB500-511 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. I am not familiar enough with your thrombopenia model to say for certain if this antibody will work for you studies, but this should give you some insight as to whether or not it is suitable for your studies. Furthermore, this antibody does contain 15mM sodium azide which would have to be removed before injection. We have kits that will allow you to do this clean-up, if you are interested.
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