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CD42b/GPIb alpha Antibody (HIP1) - BSA Free

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Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) APC.
Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human platelets with anti-CD42b (HIP1) PE.
Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) purified / GAM-APC.
Surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
HIP1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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CD42b/GPIb alpha Antibody (HIP1) - BSA Free Summary

Additional Information
Clone HIP1 was used by HLDA to establish CD designation.
Immunogen
Peripheral blood mononuclear cells of a patient suffering with CLL.
Specificity
The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GP1BA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
Application Notes
Functional Application: The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. This antibody is CyTOF ready.
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB500-511 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31467123).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

  • Antigen CD42b-alpha
  • BP1BA
  • BSS
  • CD42b antigen
  • CD42b
  • CD42b-alpha
  • glycoprotein Ib (platelet), alpha polypeptide
  • Glycoprotein Ibalpha
  • GP1B
  • GP1BA
  • GPIb alpha
  • GP-Ib alpha
  • GPIbA
  • GPIb-alpha
  • MGC34595
  • platelet glycoprotein Ib alpha chain
  • platelet membrane glycoprotein 1b-alpha subunit

Background

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD42b/GPIb alpha Antibody (NB500-511)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Mouse
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FAQs for CD42b/GPIb alpha Antibody (NB500-511). (Showing 1 - 1 of 1 FAQs).

  1. I want to buy the anti- CD42b/GPIbA monclonal antibody, which is used to inject into the mouse to make the animal model of thrombopenia. Can the anti- CD42b/GPIbA antibody(NB500-511) be used in this experiment?
    • NB500-511 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. I am not familiar enough with your thrombopenia model to say for certain if this antibody will work for you studies, but this should give you some insight as to whether or not it is suitable for your studies. Furthermore, this antibody does contain 15mM sodium azide which would have to be removed before injection. We have kits that will allow you to do this clean-up, if you are interested.

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Bioinformatics

Gene Symbol GP1BA
Entrez