Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by ...read more
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD36/SR‑B3 ...read more
Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Goat Anti-Human ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD36/SR-B3 Antibody [Unconjugated]
BDPLT10
CD_antigen: CD36
CD36 antigen (collagen type I receptor, thrombospondin receptor)
CD36 antigen
CD36 molecule (thrombospondin receptor)
CD36
CHDS7
cluster determinant 36
Collagen R
FAT
Fatty acid translocase
Glycoprotein IIIb
GP3B
GP4
GPIIIb
GPIV
PAS IV
PAS-4 protein
PAS-4
PASIV
Platelet collagen receptor
platelet glycoprotein 4
SCARB3
scavenger receptor class B, member 3
SRB3
SR-B3
Thrombospondin R
Thrombospondin receptor
Background
CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.
Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
Khoury, J. et al. (2003) J. Exp. Med. 197:1657.
Husemann, J. et al. (2002) Glia 40:195.
Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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