CD36 Antibody (D-2712) - BSA Free

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IF/IHC, WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NB110-59724	Applications	Species
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB, Mouse) Details: Citation using the HRP version of this antibody.	WB	Mouse
Yuan C, Guo X, Zhou Q et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing beta-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 2019-01-01 [PMID: 30389579] (Mouse)		Mouse
Kuhnel E, Kleff V, Stojanovska V et al. PlacentalSpecific Overexpression of sFlt1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model. Journal of Cellular Biochemistry. 2016-11-09 [PMID: 27859593] (WB, Mouse)	WB	Mouse
Nusken E, Gellhaus A, Kuhnel E et al. Increased Rat Placental Fatty Acid, But Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J. Cell. Biochem. 2015-11-21 [PMID: 26590355] (WB, Rat)	WB	Rat
Georgiou, D K, Dagnino-Acosta, A Et al. Ca2+ Binding/Permeation via Calcium Channel, CaV1.1, Regulates the Intracellular Distribution of the Fatty Acid Transport Protein, CD36, and Fatty Acid Metabolism. J Biol Chem 2015-09-25 [PMID: 26245899] (IF/IHC, Mouse) Details: Citation using the DyLight 550 format of this antibody.	IF/IHC	Mouse
Gong T, Wang Q, Lin Z et al. Endoplasmic reticulum (ER) stress inhibitor salubrinal protects against ceramide-induced SH-SY5Y cell death. Biochem Biophys Res Commun. 2012-01-01 [PMID: 22935424]
Feldhahn N, Arutyunyan A, Stoddart S et al. Environment-mediated drug resistance in Bcr/Abl-positive acute lymphoblastic leukemia Oncoimmunology 2012-08-01 [PMID: 22934254] (WB, Mouse)	WB	Mouse
During A, Doraiswamy S, Harrison EH. Xanthophylls are preferentially taken up compared with {beta}-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res;49(8):1715-1724. 2008-01-01 [PMID: 18424859] (WB, Human)	WB	Human
Duan SZ, Ivashchenko CY, Russell MW, Milstone DS, Mortensen RM. Cardiomyocyte-specific knockout and agonist of peroxisome proliferator-activated receptor-gamma both induce cardiac hypertrophy in mice. Circ Res;97(4):372-9. 2005-08-19 [PMID: 16051889] (WB, IF/IHC, Mouse)	WB, IF/IHC	Mouse
Chen YJ, Lo YH, Chen YT et al. Magnesium lithospermate B improves metabolic changes in high-fat diet-fed rats with metabolic syndrome Journal of Functional Foods. 2015-04-01 (WB, Rat) Details: CD36/SR-B3 antibody was used for WB application on lysates of liver tissue from rats that were fed different diet regimns as - normal diet control/NC, high-fat diet control/HC or HC+ MLB/Magnesium lithospermate B (MLB5-20/HC+5-20?mg/kg/day of MLB). Primary antibody was used at 1:1000 dilution in 5% skim milk and detected with HRP-conjugated secondary antibody - chemiluminescent HRP substrate (data shown in Fig. 4A).	WB	Rat
Febbraio M et al. Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest;105(8):1049-56. 2000-04-01 [PMID: 10772649] (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Jason Plummer	WB	Rat	11/28/2011	View Summary Application Western Blot Sample Tested Liver whole tissue homogenate, Sample Amount: 50ug Species Rat

Array NB110-59724

• CD36 Antibodies
• CD36 ELISA Kits
• CD36 Lysates
• CD36 Proteins