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CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

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Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of bladder cancer tissues using IL2RA mouse mAb with DAB staining.
Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of rectum cancer tissues using IL2RA mouse mAb with DAB staining.
ELISA: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1B5D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37349-0.025ml
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CD25/IL-2R alpha Antibody (1B5D12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD25/IL-2R alpha (AA: 34-139) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL2RA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2RA