![Immunohistochemistry: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Human skin. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.](http://images.novusbio.com/fullsize/CD1a-Antibody-O10-IHC-Prediluted-Immunohistochemistry-NBP2-45341-img0003.jpg "Immunohistochemistry: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Human skin. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | IHC |
| Clone | O10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Additional Information | Clone O10 was used by HLDA to establish CD designation. |
| Immunogen | Human thymus cells |
| Localization | Cell surface |
| Marker | Mature Langerhans Cells Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD1A |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD1a Antibody (NBP2-45341)Find related products by research area.
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| Gene Symbol | CD1A |
![Immunohistochemistry-Paraffin: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10).](http://images.novusbio.com/fullsize/CD1a-Antibody-O10-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45341-img0002.jpg "Immunohistochemistry-Paraffin: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")