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CD1a Antibody (703217) [Unconjugated]

Images

 
MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1a Monoclonal Antibody (Catalog # MAB7076, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more
CD1a was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CD1a Monoclonal Antibody (Catalog # MAB7076) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
CD1a was detected in immersion fixed paraffin-embedded sections of Human Skin using Mouse Anti-Human CD1a Monoclonal Antibody (Catalog # MAB7076) at 3 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
703217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CD1a Antibody (703217) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1a
Asp19-Val300 (predicted)
Accession # P06126
Specificity
Detects human CD1a in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD1d is observed and no cross-reactivity with with rhCD1b or rhCD1e is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD1A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 3-15 ug/mL
Publications
Read Publication using MAB7076.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD1a Antibody (703217) [Unconjugated]

  • CD1
  • CD1a antigen
  • CD1A antigen, a polypeptide
  • CD1a molecule
  • CD1a
  • cluster of differentiation 1 A
  • cortical thymocyte antigen CD1A
  • differentiation antigen CD1-alpha-3
  • epidermal dendritic cell marker CD1a
  • FCB6
  • hTa1 thymocyte antigen
  • HTA1
  • R4
  • T6
  • T-cell surface antigen T6/Leu-6
  • T-cell surface glycoprotein CD1a

Background

CD1a is a 49 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1a contains one Ig-like domain in its extracellular region. It is expressed by most nonhuman mammals but not by mice or rats. Complexes of CD1a with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, and Langerhans cells. CD1a is a target of autoreactive Th22 helper T cells in the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD1a Antibody (MAB7076)(1)

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Bioinformatics

Gene Symbol CD1A
Uniprot