Home » CD161/NK1.1 » CD161/NK1.1 Antibodies » CD161/NK1.1 Antibody (PK136) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

 Submit a Review

Images

 
(
Enlarge)
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Mouse. Image from the standard format of this antibody.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Mouse. Image from the standard format of this antibody.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Analysis using the PE/Cy7 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC and ...read more
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC and ...read more
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - C57BL/6 mouse splenocytes stained with purified PK136, followed by anti-mouse IgG2a FITC Image from the standard format of this antibody.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Mouse splenocytes were stained with CD49b (DX5) APC and purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), ...read more
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Staining of C57BL/6 mouse splenocytes with Anti-Mouse NK-1.1 PE and Anti-Mouse CD49b (Integrin alpha 2) FITC. Total viable cells were used ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
PK136
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-77528AF350
Alexa Fluor 405
NB100-77528AF405
Alexa Fluor 488
NB100-77528AF488
Alexa Fluor 532
NB100-77528AF532
Alexa Fluor 594
NB100-77528AF594
Alexa Fluor 647
NB100-77528AF647
Alexa Fluor 700
NB100-77528AF700
Alexa Fluor 750
NB100-77528AF750
Allophycocyanin
NB100-77528APC
Allophycocyanin/Cy7
NB100-77528APCCY7
Biotin
NB100-77528B
DyLight 350
NB100-77528UV
DyLight 405
NB100-77528V
DyLight 488
NB100-77528G
DyLight 550
NB100-77528R
DyLight 594
NB100-77528DL594
DyLight 650
NB100-77528C
DyLight 680
NB100-77528FR-500ul
DyLight 755
NB100-77528IR
FITC
NB100-77528F
HRP
NB100-77528H
Janelia Fluor 549
NB100-77528JF549
Janelia Fluor 646
NB100-77528JF646
PE
NB100-77528PE
PE/Atto594
NB100-77528PEATT594
PE/Cy5.5
NB100-77528PECY55
PE/Cy7
NB100-77528PECY7
PerCP
NB100-77528PCP-500ul
Unconjugated
NB100-77528
mFluor Violet 450 SE
NB100-77528MFV450
mFluor Violet 500 SE
NB100-77528MFV500
mFluor Violet 610 SE
NB100-77528MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-77528
BSA Free
NB100-77528SS
BSA Free
NB100-77528
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CD161/NK1.1 Antibody (PK136) - Azide and BSA Free Summary

Immunogen
NK-1+ cells from mouse spleen and bone marrow
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
Klrb1c
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1.0 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:20-1:1000. Use reported in scientific literature (PMID 21118981)
  • Immunoprecipitation 1:20-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 ug/1 x10^6 cells in 100 ul It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

  • CD161 antigen
  • CD161
  • CD161/NK1.1
  • CD161c
  • CLEC5BC-type lectin domain family 5 member B
  • HNKR-P1a
  • killer cell lectin-like receptor subfamily B member 1
  • killer cell lectin-like receptor subfamily B, member 1
  • Klrb1c
  • Ly55c
  • Ly-59
  • Natural killer cell surface protein P1A
  • NKR-P1
  • NKR-P1AMGC138614
  • NKRP1ANKR

Background

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Substance P
Antibody (266815)
MAB4375
Immunohistochemistry Substance P Antibody (266815)
(1 Review)
(9 Publications)
Species: Hu, Mu, Rt
Applications: IHC
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
TFF2 Antibody
(366508) [Unconjugate
MAB4077
Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(450 Publications)
Species: Hu
Applications: BA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
CD3 Antibody (SP7)
NB600-1441
Immunohistochemistry-Frozen CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
(3 Reviews)
(44 Publications)
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
CD161 Antibody
(14F1F11) - BSA Free
NBP2-14845
Immunohistochemistry-Paraffin CD161 Antibody (14F1F11) - BSA Free
Immunohistochemistry-Paraffin CD161 Antibody (14F1F11) - BSA Free
Western Blot CD161 Antibody (14F1F11) - BSA Free
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(810 Publications)
Species: Hu
Applications: BA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Neurokinin B
Antibody - BSA Free
NB300-201
Immunohistochemistry Neurokinin B Antibody - BSA Free
Immunohistochemistry Neurokinin B Antibody - BSA Free
Immunohistochemistry Neurokinin B Antibody - BSA Free
(2 Reviews)
(25 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD1a Antibody
(NA1/34-HLK)
NB100-64852
Flow Cytometry CD1a Antibody (NA1/34-HLK)
Flow Cytometry CD1a Antibody (NA1/34-HLK)
Immunohistochemistry-Frozen CD1a Antibody (NA1/34-HLK)
(1 Review)
(8 Publications)
Species: Hu
Applications: Flow, IF, IHC, IHC-Fr
CD1c/BDCA-1
Antibody (OTI2F4)
NBP2-46123
Immunocytochemistry/ Immunofluorescence CD1c/BDCA-1 Antibody (OTI2F4)
Immunohistochemistry CD1c/BDCA-1 Antibody (OTI2F4)
Immunohistochemistry CD1c/BDCA-1 Antibody (OTI2F4)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CD1b Antibody
(737249) [Unconjugate
MAB7446
Flow Cytometry CD1b Antibody (737249) [Unconjugated]
(1 Publication)
Species: Hu
Applications: CyTOF-ready, Flow
CD45 Antibody
[Unconjugated]
AF114
Immunohistochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]
(1 Review)
(70 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Publications for CD161/NK1.1 Antibody (NBP2-80631) (0)

There are no publications for CD161/NK1.1 Antibody (NBP2-80631).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD161/NK1.1 Antibody (NBP2-80631) (0)

There are no reviews for CD161/NK1.1 Antibody (NBP2-80631). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD161/NK1.1 Antibody (NBP2-80631) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD161/NK1.1 Products

Array NBP2-80631

Research Areas for CD161/NK1.1 Antibody (NBP2-80631)

Find related products by research area.

Blogs on CD161/NK1.1

There are no specific blogs for CD161/NK1.1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD161/NK1.1 Antibody (PK136) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol Klrb1c