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CD161/NK1.1 Antibody (PK136) [mFluor Violet 450 SE]

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CD161/NK1.1 Antibody (PK136) [mFluor Violet 450 SE] [NB100-77528MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.
CD161/NK1.1 Antibody (PK136) [mFluor Violet 450 SE] [NB100-77528MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
PK136
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-77528AF350
Alexa Fluor 405
NB100-77528AF405
Alexa Fluor 488
NB100-77528AF488
Alexa Fluor 532
NB100-77528AF532
Alexa Fluor 594
NB100-77528AF594
Alexa Fluor 647
NB100-77528AF647
Alexa Fluor 700
NB100-77528AF700
Alexa Fluor 750
NB100-77528AF750
Allophycocyanin
NB100-77528APC
Allophycocyanin/Cy7
NB100-77528APCCY7
Biotin
NB100-77528B
DyLight 350
NB100-77528UV
DyLight 405
NB100-77528V
DyLight 488
NB100-77528G
DyLight 550
NB100-77528R
DyLight 594
NB100-77528DL594
DyLight 650
NB100-77528C
DyLight 680
NB100-77528FR-500ul
DyLight 755
NB100-77528IR
FITC
NB100-77528F
HRP
NB100-77528H
Janelia Fluor 549
NB100-77528JF549
Janelia Fluor 646
NB100-77528JF646
PE
NB100-77528PE
PE/Atto594
NB100-77528PEATT594
PE/Cy5.5
NB100-77528PECY55
PE/Cy7
NB100-77528PECY7
PerCP
NB100-77528PCP-500ul
Unconjugated
NB100-77528
mFluor Violet 500 SE
NB100-77528MFV500
mFluor Violet 610 SE
NB100-77528MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-77528
BSA Free
NB100-77528SS
BSA Free
NB100-77528
Azide and BSA Free
NBP2-80631
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CD161/NK1.1 Antibody (PK136) [mFluor Violet 450 SE] Summary

Immunogen
NK-1+ cells from mouse spleen and bone marrow
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
Klrb1c
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD161/NK1.1 Antibody (PK136) [mFluor Violet 450 SE]

  • CD161 antigen
  • CD161
  • CD161/NK1.1
  • CD161c
  • CLEC5BC-type lectin domain family 5 member B
  • HNKR-P1a
  • killer cell lectin-like receptor subfamily B member 1
  • killer cell lectin-like receptor subfamily B, member 1
  • Klrb1c
  • Ly55c
  • Ly-59
  • Natural killer cell surface protein P1A
  • NKR-P1
  • NKR-P1AMGC138614
  • NKRP1ANKR

Background

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol Klrb1c