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Recombinant Human CCL8/MCP-2 Protein

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SDS-Page: Human CCL8/MCP-2 Protein [NBP2-34969]
SDS-Page: Human CCL8/MCP-2 Protein [NBP2-34969]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-34969-1mg
Carrier-Free
NBP2-34969-100ug
Carrier-Free
NBP2-34969-500ug
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Recombinant Human CCL8/MCP-2 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to CCL8/MCP-2 Source: E. coli

Uniprot ID: P80075

Amino Acid Sequence: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
Details of Functionality
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CCL8
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CCL8/MCP-2 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
8.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human CCL8/MCP-2 Protein

  • C-C motif chemokine 8
  • CCL8
  • chemokine (C-C motif) ligand 8
  • HC14
  • MCP2
  • MCP-2
  • MCP-2/CCL8
  • member 8 (monocyte chemotactic protein 2)
  • monocyte chemoattractant protein 2
  • SCYA10
  • small-inducible cytokine A8

Background

Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1- beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
CCL8/MCP-2 Protein
NBP2-34969
Recombinant Human CCL8/MCP-2 Protein
Species: Hu
Applications: PAGE, Bioactivity

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Bioinformatics

Gene Symbol CCL8
Uniprot