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CapG Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody (A) NBP1-90214 shows similar ...read more
Independent Antibodies: Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).
Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP1-90214] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in cells in lamina propria.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CapG Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLF
Predicted Species
Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CapG Protein (NBP1-90214PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CapG Antibody

  • actin-regulatory protein CAP-G
  • AFCP
  • CapG
  • capping protein (actin filament), gelsolin-like
  • gelsolin-like capping protein
  • macrophage capping protein
  • macrophage-capping protein
  • MCPActin regulatory protein CAP-G

Background

Actin Regulatory Protein encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CapG Antibody (NBP1-90214) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAPG