Home » CCL7/MCP-3/MARC » CCL7/MCP-3/MARC Antibodies » CCL7/MCP-3/MARC Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CCL7/MCP-3/MARC Antibody [Unconjugated]

 (15 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND50 is typically 7.5-45 µg/mL.
Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF456
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-456-SP
100% Guarantee on every product

CCL7/MCP-3/MARC Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL7/MCP-3/MARC
Gln24-Pro97
Accession # Q03366
Specificity
Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL7
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 15-45 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-456-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL7/MCP-3/MARC Antibody [Unconjugated]

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

  1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
  2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
  3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(250 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(99 Publications)
Species: Hu
Applications: ELISA
CCL8/MCP-2 [Biotin]
DY790
N/A CCL8/MCP-2 [Biotin]
(11 Publications)
Species: Mu
Applications: ELISA
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CCL13/MCP-4
[Biotin]
DY327
N/A CCL13/MCP-4 [Biotin]
(12 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
CCR2 Antibody
(48607) [Unconjugated
MAB150
Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]
Flow Cytometry CCR2 Antibody (48607) [Unconjugated]
(43 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(151 Publications)
Species: Hu
Applications: ELISA
CD46 Antibody
[Unconjugated]
AF2005
Immunohistochemistry CD46 Antibody [Unconjugated]
Western Blot CD46 Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
CapG Antibody
NBP1-90214
Immunocytochemistry/ Immunofluorescence CapG Antibody
Western Blot CapG Antibody
Immunohistochemistry-Paraffin CapG Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(180 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(631 Publications)
Species: Mu
Applications: ELISA
CCR3 Antibody
(61828)
MAB155
Immunohistochemistry CCR3 Antibody (61828)
Neutralization CCR3 Antibody (61828)
(20 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CCR1 Antibody
(53504) [Unconjugated
MAB145
Flow Cytometry CCR1 Antibody (53504) [Unconjugated]
(1 Review)
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
CXCR1/IL-8RA
Antibody (42705) [Unc ...
MAB330
Immunohistochemistry CXCR1/IL-8RA Antibody (42705) [Unconjugated]
Immunohistochemistry CXCR1/IL-8RA Antibody (42705) [Unconjugated]
Flow Cytometry CXCR1/IL-8RA Antibody (42705) [Unconjugated]
(50 Publications)
Species: Hu
Applications: CyTOF-reported, Flow, IHC, Neut
CXCL9/MIG Antibody
[Unconjugated]
AF-492-NA
Neutralization CXCL9/MIG Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]
Western Blot CXCL9/MIG Antibody [Unconjugated]
(31 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA

Publications for CCL7/MCP-3/MARC Antibody (AF-456-NA)(15)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Bioassay, Flow Cytometry, In Vivo, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(1)
In Vivo
(1)
Neutralization
(4)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(9)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF-456-NA Applications Species
Gregory P. Takacs, Christian J. Kreiger, Defang Luo, Guimei Tian, Julia S. Garcia, Loic P. Deleyrolle et al. Glioma-derived CCL2 and CCL7 mediate migration of immune suppressive CCR2+/CX3CR1+ M-MDSCs into the tumor microenvironment in a redundant manner Frontiers in Immunology 2023-01-04 [PMID: 36685592]
T Komori, Y Morikawa Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise The Journal of Biological Chemistry, 2022-11-09;0(0):102686. 2022-11-09 [PMID: 36370846] (Western Blot, Mouse) Western Blot Mouse
Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae-I Moon, Eunji Lee et al. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF kappa B activation Cellular and Molecular Life Sciences 2022-03-01 [PMID: 35218410]
C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype Journal of Cellular and Molecular Medicine, 2021-06-29;0(0):. 2021-06-29 [PMID: 34189838] (Neutralization, Mouse) Neutralization Mouse
SL Kurtz, R De Pascali, AI Meierovics, KL Elkins Deficiency in CCR2 increases susceptibility of mice to infection with an intracellular pathogen, Francisella tularensis LVS, but does not impair development of protective immunity PLoS ONE, 2021-03-24;16(3):e0249142. 2021-03-24 [PMID: 33760886] (Neutralization, Mouse) Neutralization Mouse
Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J. Stewart, Nicolas Brouilly et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages Immunity 2020-07-01 [PMID: 32562599]
Qi D, Wei M, Jiao S et al. Hypoxia inducible factor 1 alpha in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment Cell Death Dis 7/18/2019 [PMID: 31320613]
J He, Y Song, G Li, P Xiao, Y Liu, Y Xue, Q Cao, X Tu, T Pan, Z Jiang, X Cao, L Lai, Q Wang Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation J. Clin. Invest., 2019-06-27;130(0):. 2019-06-27 [PMID: 31246581] (Bioassay, Mouse) Bioassay Mouse
K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia Cytokine, 2019-04-16;119(0):202-213. 2019-04-16 [PMID: 31003094] (In Vivo, Mouse) In Vivo Mouse
Girkin J, Hatchwell L, Foster P, Johnston S, Bartlett N, Collison A, Mattes J CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection. J Immunol, 2015-04-06;194(10):4924-30. 2015-04-06 [PMID: 25847975] (Neutralization, Mouse) Neutralization Mouse
Show All 15 Publications.

Reviews for CCL7/MCP-3/MARC Antibody (AF-456-NA) (0)

There are no reviews for CCL7/MCP-3/MARC Antibody (AF-456-NA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL7/MCP-3/MARC Antibody (AF-456-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL7/MCP-3/MARC Products

Blogs on CCL7/MCP-3/MARC

There are no specific blogs for CCL7/MCP-3/MARC, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL7/MCP-3/MARC Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL7
Uniprot