CCL4/MIP-1 beta Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: ELISA Development, Flow Cytometry, ICC, IHC, IHC-P, Immunohistochemistry, Neutralization.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using AF-451-NA	Applications	Species
Gern, BH;Klas, JM;Foster, KA;Cohen, SB;Plumlee, CR;Duffy, FJ;Neal, ML;Halima, M;Gustin, AT;Diercks, AH;Aderem, A;Gale, M;Aitchison, JD;Gerner, MY;Urdahl, KB; CD4-mediated immunity shapes neutrophil-driven tuberculous pathology bioRxiv : the preprint server for biology 2024-04-15 [PMID: 38659794] (Flow Cytometry, Immunohistochemistry, Mouse, Transgenic Mouse)	Flow Cytometry, Immunohistochemistry	Mouse, Transgenic Mouse
Van Anh Do-Thi, Sang Min Park, Song Mi Park, Hye Jin Jeong, Geunyoung Cho, Hyun-Jung An et al. IL9 Polarizes Macrophages to M1 and Induces the Infiltration of Antitumor Immune Cells via MIP-1 and CXCR3 Chemokines Cancer Research Communications 2023-01-18 [PMID: 36968220]
Jessica M. Rosin, Nikol Tretiakov, Emily Hanniman, Kiana Hampton, Deborah M. Kurrasch Gestational Bisphenol A Exposure Impacts Embryonic Hypothalamic Microglia Numbers, Ramification, and Phagocytic Cups Frontiers in Neuroscience 2022-02-18 [PMID: 35250464]
AM Osman, Y Sun, TC Burns, L He, N Kee, N Oliva-Vila, A Alevyzaki, K Zhou, L Louhivuori, P Uhlén, E Hedlund, C Betsholtz, VM Lauschke, J Kele, K Blomgren Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain Cell Rep, 2020-06-02;31(9):107699. 2020-06-02 [PMID: 32492415] (IHC, Mouse)	IHC	Mouse
JM Williford, J Ishihara, A Ishihara, A Mansurov, P Hosseinchi, TM Marchell, L Potin, MA Swartz, JA Hubbell Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy Sci Adv, 2019-12-11;5(12):eaay1357. 2019-12-11 [PMID: 31844672] (IHC, Mouse)	IHC	Mouse
Timothy R. Hammond, Connor Dufort, Lasse Dissing-Olesen, Stefanie Giera, Adam Young, Alec Wysoker et al. Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes Immunity 2019-01-01 [PMID: 30471926]
VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Sci Rep, 2017-04-20;7(0):46514. 2017-04-20 [PMID: 28425451] (Neutralization, Mouse, Rat)	Neutralization	Mouse, Rat
Chang-Hyun Song, Osamu Honmou, Hidefumi Furuoka, Motohiro Horiuchi Identification of Chemoattractive Factors Involved in the Migration of Bone Marrow-Derived Mesenchymal Stem Cells to Brain Lesions Caused by Prions Journal of Virology 2011-11-01 [PMID: 21813601]
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J. Immunol., 2008-11-01;181(9):6384-93. 2008-11-01 [PMID: 18941229] (IHC-P, Mouse)	IHC-P	Mouse
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. J. Exp. Med., 2008-08-11;205(9):2075-84. 2008-08-11 [PMID: 18695004] (Neutralization, Mouse)	Neutralization	Mouse
Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses. Diabetes, 2007-03-01;56(3):809-17. 2007-03-01 [PMID: 17327452] (Neutralization, Mouse)	Neutralization	Mouse
Cardona AE, Gonzalez PA, Teale JM CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Infect. Immun., 2003-05-01;71(5):2634-42. 2003-05-01 [PMID: 12704138] (ICC, Mouse)	ICC	Mouse
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. J. Immunol., 2000-03-01;164(5):2650-9. 2000-03-01 [PMID: 10679105] (ELISA Development, Rat)	ELISA Development	Rat
Show All 14 Publications. Collapse Publications.

Array AF-451-NA

• CCL4/MIP-1 beta Antibodies
• CCL4/MIP-1 beta ELISA Kit
• CCL4/MIP-1 beta Lysate
• CCL4/MIP-1 beta Proteins
• CCL4/MIP-1 beta Proteins and Enzymes
• CCL4/MIP-1 beta ELISAs