Home » CCL19/MIP-3 beta » CCL19/MIP-3 beta Antibodies » CCL19/MIP-3 beta Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CCL19/MIP-3 beta Antibody [Unconjugated]

 (15 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Mouse CCL19/MIP‑3 beta (Catalog #440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 μg/mL.
Recombinant Mouse CCL19/MIP‑3 beta (Catalog #440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through ...read more
CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. ...read more
CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF880
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF880-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CCL19/MIP-3 beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ccl19
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.8-4 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF880 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody [Unconjugated]

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta . MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL21/6Ckine
[Unconjugated]
366-6C
CCL21/6Ckine [Unconjugated]
(33 Publications)
Species: Hu
Applications: BA
CCR7 Antibody
(150503) [Unconjugate
MAB197
Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]
Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]
Neutralization CCR7 Antibody (150503) [Unconjugated]
(1 Review)
(102 Publications)
Species: Hu
Applications: CyTOF-reported, Dual ISH-IHC, Flow, ICC, IHC, Neut
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
CXCL12/SDF-1 alpha
[Unconjugated]
350-NS
SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]
Bioactivity CXCL12/SDF-1 alpha [Unconjugated]
(180 Publications)
Species: Fe, Hu, RM
Applications: BA, BA
CXCL13/BLC/BCA-1
Antibody [Unconjugate ...
AF470
Immunohistochemistry CXCL13/BLC/BCA-1 Antibody [Unconjugated]
Neutralization CXCL13/BLC/BCA-1 Antibody [Unconjugated]
(2 Reviews)
(58 Publications)
Species: Mu
Applications: IHC, Neut, WB
CCL20/MIP-3 alpha
[HRP]
DM3A00
N/A CCL20/MIP-3 alpha [HRP]
N/A CCL20/MIP-3 alpha [HRP]
N/A CCL20/MIP-3 alpha [HRP]
(52 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(251 Publications)
Species: Hu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CCRL2/LCCR
Antibody (498321)
MAB5519
Flow Cytometry CCRL2/LCCR Antibody (498321)
Immunocytochemistry CCRL2/LCCR Antibody (498321)
(1 Publication)
Species: Mu
Applications: CyTOF-ready, Flow, ICC
CCR6 Antibody
(53103)
MAB195
Immunohistochemistry CCR6 Antibody (53103)
(37 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(152 Publications)
Species: Hu
Applications: ELISA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(290 Publications)
Species: Mu
Applications: ELISA
CCL17/TARC [HRP]
MCC170
N/A CCL17/TARC [HRP]
N/A CCL17/TARC [HRP]
(45 Publications)
Species: Mu
Applications: ELISA
CXCR4 Antibody
(44716) [Unconjugated
MAB172
Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]
Neutralization CXCR4 Antibody (44716) [Unconjugated]
Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]
(2 Reviews)
(87 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA

Publications for CCL19/MIP-3 beta Antibody (AF880)(15)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Flow Cytometry
(2)
IHC
(2)
IHC-Fr
(4)
IHC-P
(1)
Neutralization
(3)
All Applications
Filter By Species
Mouse
(11)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF880 Applications Species
J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Flow Cytometry, Mouse) Flow Cytometry Mouse
V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses Cell Reports, 2022-04-19;39(3):110727. 2022-04-19 [PMID: 35443187] (IHC, Mouse) IHC Mouse
Juana Serrano-Lopez, Shailaja Hegde, Sachin Kumar, Josefina Serrano, Jing Fang, Ashley M Wellendorf et al. Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics eLife 2021-04-08 [PMID: 33830019]
M Chen, RR Reed, AP Lane Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell, 2019-09-12;25(4):501-513.e5. 2019-09-12 [PMID: 31523027] (IHC-Fr, Neutralization, Mouse) IHC-Fr, Neutralization Mouse
Alice E. Denton, Edward J. Carr, Lukasz P. Magiera, Andrew J.B. Watts, Douglas T. Fearon Embryonic FAP+ lymphoid tissue organizer cells generate the reticular network of adult lymph nodes Journal of Experimental Medicine 2019-10-07 [PMID: 31324739]
AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia J Am Heart Assoc, 2017-03-08;6(3):. 2017-03-08 [PMID: 28275068] (IHC-Fr, Mouse) IHC-Fr Mouse
Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda et al. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney JCI Insight 2016-10-05 [PMID: 27699223]
Jing Hua, William Stevenson, Thomas H. Dohlman, Takenori Inomata, Maryam Tahvildari, Narghes Calcagno et al. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis Investigative Opthalmology & Visual Science 2016-04-01 [PMID: 27031839]
Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun, 2014-05-12;82(8):3098-112. 2014-05-12 [PMID: 24818662] (IHC, Mouse) IHC Mouse
Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B. J. Immunol., 2010-04-19;184(10):5571-81. 2010-04-19 [PMID: 20404272] (Neutralization, Mouse) Neutralization Mouse
Show All 15 Publications.

Reviews for CCL19/MIP-3 beta Antibody (AF880) (0)

There are no reviews for CCL19/MIP-3 beta Antibody (AF880). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL19/MIP-3 beta Antibody (AF880) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL19/MIP-3 beta Products

Blogs on CCL19/MIP-3 beta

There are no specific blogs for CCL19/MIP-3 beta, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL19/MIP-3 beta Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol Ccl19
Uniprot