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CCL19/MIP-3 beta Antibody (866202)

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Recombinant Rat CCL19/MIP‑3 beta (Catalog # 7800-M3) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL19/MIP‑3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB7800). The ND50 is typically 0.4‑2.4 μg/mL.
Recombinant Rat CCL19/MIP‑3 beta (Catalog # 7800-M3) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications B/N
Clone
866202
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7800-SP
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CCL19/MIP-3 beta Antibody (866202) Summary

Immunogen
E. coli-derived recombinant rat CCL19/MIP‑3 beta
Gly26-Ser108
Accession # NP_001102131
Specificity
Detects rat CCL19/MIP‑3 beta in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL19
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.4-2.4 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody (866202)

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19 (also known as Mip-3 beta , ELC, CK beta -11, Scya19 and Exodus-3) is a 9 kDa secreted member of the CC family of chemotactic cytokines (1-4). Cells known to express CCL19 are limited in number, and include activated monocytes (5), stromal cells in nodal T zones (6), CD8+ dendritic cells (DC) (6), vascular endothelial cells (7), visceral smooth muscle and mast cells (8), and thymic stromal cells (9).  Rat CCL19 is synthesized as a precursor that is 1108 amino acids (aa) in length. Mature rat CCL19 shares 72% and 89% aa sequence identity with human and mouse CCL19, respectively. CCL19 is known to bind to CCR7 (3, 4), CCLR2/CRAM (L-CCR in rodent) (10, 11) and CCX-CKR (12), with the last two receptors representing scavenger, or chemokine-sink receptors.
  1. Sharma, M. (2010) Crit. Rev. Biotechnol. 30:1.
  2. Blanchet, X. et al. (2012) Front. Immunol. 3:175.
  3. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
  4. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  5. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  6. Luther, S.A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12694.
  7. Alt, C. et al. (2002) Eur. J. Immunol. 32:2133.
  8. Kaur, D. et al. (2006) Am. J. Respir. Crit. Care Med. 174:1179.
  9. Takamura, K. et al. (2007) J. Immunol. 179:5897.
  10. Leick, M. et al. (2009) Immunology 129:536.
  11. Yoshimura, T. & J.J. Oppenheim (2011) Exp. Cell Res. 317:674.
  12. Comerford, I. et al. (2006) Eur. J. Immunol. 36:1904

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCL19
Uniprot