Home » CCL13/MCP-4 » CCL13/MCP-4 Antibodies » CCL13/MCP-4 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CCL13/MCP-4 Antibody [Unconjugated]

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Human CCL13/MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP‑4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL13/MCP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF327). The ND50 is typically 15‑40 µg/mL.
Recombinant Human CCL13/MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF327
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF327-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CCL13/MCP-4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL13/MCP-4
Gln24-Thr98
Accession # Q99616
Specificity
Detects human CCL13/MCP-4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL13
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 15-40 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF327 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL13/MCP-4 Antibody [Unconjugated]

  • C-C motif chemokine 13
  • CCL13
  • chemokine (C-C motif) ligand 13
  • CKb10
  • MCP4
  • MCP-4
  • MCP-4Monocyte chemoattractant protein 4
  • MGC17134
  • NCC-1Monocyte chemotactic protein 4
  • NCC1Small-inducible cytokine A13
  • new CC chemokine 1
  • SCYA13CK-beta-10
  • SCYL1
  • small inducible cytokine subfamily A (Cys-Cys), member 13

Background

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CCL7/MCP-3/MARC
Antibody [Unconjugate
AF-456-NA
Neutralization CCL7/MCP-3/MARC Antibody [Unconjugated]
(15 Publications)
Species: Mu
Applications: Neut, WB
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(256 Publications)
Species: Hu
Applications: ELISA
CCL8/MCP-2 [Biotin]
DY790
N/A CCL8/MCP-2 [Biotin]
(11 Publications)
Species: Mu
Applications: ELISA

CCL24/Eotaxin-2/MPIF-2
DY528
N/A CCL24/Eotaxin-2/MPIF-2 [Biotin]
(35 Publications)
Species: Mu
Applications: ELISA
CCR3 Antibody
(61828)
MAB155
Immunohistochemistry CCR3 Antibody (61828)
Neutralization CCR3 Antibody (61828)
(20 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
CapG Antibody
NBP1-90214
Immunocytochemistry/ Immunofluorescence CapG Antibody
Western Blot CapG Antibody
Immunohistochemistry-Paraffin CapG Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CD46 Antibody
(344519) [Unconjugate
MAB2005
N/A CD46 Antibody (344519) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CCR2 Antibody
(48607) [Unconjugated
MAB150
Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]
Flow Cytometry CCR2 Antibody (48607) [Unconjugated]
(45 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
CCL17/TARC [HRP]
MCC170
N/A CCL17/TARC [HRP]
N/A CCL17/TARC [HRP]
(45 Publications)
Species: Mu
Applications: ELISA
CCL18/PARC [Biotin]
DY394
N/A CCL18/PARC [Biotin]
(34 Publications)
Species: Hu
Applications: ELISA
CXCL9/MIG Antibody
[Unconjugated]
AF-492-NA
Neutralization CXCL9/MIG Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]
Western Blot CXCL9/MIG Antibody [Unconjugated]
(31 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut
CCR5 Antibody
(45531) [Unconjugated
MAB182
Flow Cytometry CCR5 Antibody (45531) [Unconjugated]
Neutralization CCR5 Antibody (45531) [Unconjugated]
Immunocytochemistry CCR5 Antibody (45531) [Unconjugated]
(1 Review)
(32 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, Neut
CXCR1/IL-8RA
Antibody (42705) [Unc ...
MAB330
Flow Cytometry CXCR1/IL-8RA Antibody (42705) [Unconjugated]
Neutralization CXCR1/IL-8RA Antibody (42705) [Unconjugated]
(50 Publications)
Species: Hu
Applications: CyTOF-reported, Flow, IHC, Neut
CCL26/Eotaxin-3
[Biotin]
DY346
N/A CCL26/Eotaxin-3 [Biotin]
(11 Publications)
Species: Hu
Applications: ELISA
CCL13/MCP-4
Antibody [Unconjugate
AF327
CCL13/MCP-4 Antibody [Unconjugated]
Species: Hu
Applications: WB, B/N

Publications for CCL13/MCP-4 Antibody (AF327)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC, IHC-Fr.


Filter By Application
ICC
(1)
IHC
(1)
IHC-Fr
(2)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for CCL13/MCP-4 Antibody (AF327) (0)

There are no reviews for CCL13/MCP-4 Antibody (AF327). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL13/MCP-4 Antibody (AF327) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL13/MCP-4 Products

Blogs on CCL13/MCP-4

There are no specific blogs for CCL13/MCP-4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL13/MCP-4 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL13
Uniprot