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CCL13/MCP-4 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF327
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF327
Carrier Free
AF327
Carrier Free
AF327-SP
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CCL13/MCP-4 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL13/MCP-4 (R&D Systems, Catalog # 327-P4)
Gln24-Thr98
Accession # Q99616
Specificity
Detects human CCL13/MCP-4 in ELISAs and Western blots. In sandwich immunoassays, approximately 0.3% cross-reactivity with recombinant mouse (rm) JE, recombinant human (rh) MCP-2, rhMCP-3, rmMARC, rmMCP-5, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES, and rmRANTES is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL13
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL13/MCP-4 Antibody (Catalog # MAB327)
ELISA Detection: Human CCL13/MCP-4 Biotinylated Antibody (Catalog # BAF327)
Standard: Recombinant Human CCL13/MCP-4 (Catalog # 327-P4)
Readout System
Publications
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BAF327 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL13/MCP-4 Antibody [Biotin]

  • C-C motif chemokine 13
  • CCL13
  • chemokine (C-C motif) ligand 13
  • CKb10
  • MCP4
  • MCP-4
  • MCP-4Monocyte chemoattractant protein 4
  • MGC17134
  • NCC-1Monocyte chemotactic protein 4
  • NCC1Small-inducible cytokine A13
  • new CC chemokine 1
  • SCYA13CK-beta-10
  • SCYL1
  • small inducible cytokine subfamily A (Cys-Cys), member 13

Background

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, -3, and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR2 and CCR3, both of which have been shown to bind CCL13.

  1. Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
  2. Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
  3. Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
  4. Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
  5. Heath, H. et al. (1997) J. Clin. Invest. 99:178.
  6. Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
  7. Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
  8. Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL13/MCP-4
Antibody [Biotin]
BAF327
CCL13/MCP-4 Antibody [Biotin]
Species: Hu
Applications: WB, ELISA(Det)

Publications for CCL13/MCP-4 Antibody (BAF327)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol CCL13
Uniprot