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CapG Antibody

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Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE CAPG antibody, antibody diluted at 1:1000.
Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE CAPG antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Actin Regulatory Protein Antibody [NBP2-15259] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CapG Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CapG. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus; Melanosome
Predicted Species
Rat (93%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPG
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for CapG Antibody

  • actin-regulatory protein CAP-G
  • AFCP
  • CapG
  • capping protein (actin filament), gelsolin-like
  • gelsolin-like capping protein
  • macrophage capping protein
  • macrophage-capping protein
  • MCPActin regulatory protein CAP-G

Background

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CapG Antibody
NBP2-15259
CapG Antibody
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAPG
Uniprot