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Calpain 2 Antibody

Images

 
Western Blot: Calpain 2 Antibody [NBP2-15676] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP2-15676] - Calpain 2 antibody detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 ...read more
Western Blot: Calpain 2 Antibody [NBP2-15676] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP2-15676] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Calpain 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.
Localization
Cytoplasm; Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%), Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Calpain 2 Antibody

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol CAPN2
Uniprot