Novus Biologicals products are now on bio-techne.com

Calpastatin Antibody (OTI1E7)

Images

 
Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] Staining of paraffin-embedded Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.
Flow Cytometry: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpastatin antibody, and ...read more
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Calpastatin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Calpastatin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Human colon tissue using anti-Calpastatin mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.62 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Calpastatin Antibody (OTI1E7) Summary

Immunogen
Full length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
84.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.62 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calpastatin Antibody (OTI1E7)

  • BS-17
  • Calpain inhibitor
  • calpastatin
  • MGC9402
  • Sperm BS-17 component

Background

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88204
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15671
Species: Hu, Mu, Sq
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-15671
Species: Hu, Mu, Sq
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-88204
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00008458-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP2-25162
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
NBP2-15669
Species: Mu, Rt, Ze
Applications: IHC-WhMt, IHC, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB

Publications for Calpastatin Antibody (NBP2-01819) (0)

There are no publications for Calpastatin Antibody (NBP2-01819).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Calpastatin Antibody (NBP2-01819) (0)

There are no reviews for Calpastatin Antibody (NBP2-01819). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Calpastatin Antibody (NBP2-01819) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Calpastatin Products

Research Areas for Calpastatin Antibody (NBP2-01819)

Find related products by research area.

Blogs on Calpastatin

There are no specific blogs for Calpastatin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calpastatin Antibody (OTI1E7) and receive a gift card or discount.

Bioinformatics

Gene Symbol CAST
Entrez