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FAM111B Antibody

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Western Blot: FAM111B Antibody [NBP1-86645] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: FAM111B Antibody [NBP1-86645] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FAM111B Antibody [NBP1-86645] - Staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: FAM111B Antibody [NBP1-86645] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FAM111B Antibody [NBP1-86645] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: FAM111B Antibody [NBP1-86645] - Staining of human skin shows weak to moderate nuclear positivity in the epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FAM111B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SMKTEENKSFSAMEDDQRTRPEVSKDTVMKQTHADTPVDHCLSGIRKCSSTFKLKSEVNKHETALEMQNPNLNNKECCFTFTLNGNS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAM111B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FAM111B Protein (NBP1-86645PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FAM111B Antibody

  • family with sequence similarity 111, member B

Background

FAM111B, also known as Protein FAM111B and CANP, is a 85kDa 734 amino acid protein with a shorter 81kDa 704 isoform belonging to the FAM111 family. The gene is known to interact with UBC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FAM111B