Novus Biologicals products are now on bio-techne.com

Calpain 2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human lung shows weak to moderate cytoplasmic positivity in pneumocytes.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Calpain 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Calpain 2 Protein (NBP1-88204PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Calpain 2 Antibody

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-01819
Species: Hu
Applications: Flow, IHC, IHC-P, WB
NBP2-15671
Species: Hu, Mu, Sq
Applications: ICC/IF, IHC, IHC-P, IP, WB
H00000826-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, PLA, S-ELISA, WB
NBP1-86645
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31756
Species: Hu
Applications: ICC/IF, WB
AF3844
Species: Hu, Mu
Applications: IHC
AF965
Species: Mu
Applications: IHC, Neut, WB
NBP1-88204
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF231
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
AF1029
Species: Mu
Applications: ICC, IHC, IP, WB
AF952
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
NBP1-76801
Species: Ch, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-88204
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for Calpain 2 Antibody (NBP1-88204) (0)

There are no publications for Calpain 2 Antibody (NBP1-88204).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Calpain 2 Antibody (NBP1-88204) (0)

There are no reviews for Calpain 2 Antibody (NBP1-88204). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calpain 2 Antibody (NBP1-88204) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calpain 2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CAPN2