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![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Western-Blot-NBP1-88204-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88204-img0007.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0014.jpg)
![Western Blot: Calpain 2 Antibody [NBP1-88204] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Western-Blot-NBP1-88204-img0004.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human lung shows weak to moderate cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0010.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0011.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0012.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0013.jpg)
![Western Blot: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Western-Blot-NBP2-46063-img0005.jpg "Western Blot: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.")
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0004.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0001.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0002.jpg)
![Immunohistochemistry: Calpain 2 Antibody (3G1) [NBP2-46063] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-3G1-Immunohistochemistry-NBP2-46063-img0003.jpg)
![Western Blot: Calpain 2 Antibody [NBP2-15676] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Western-Blot-NBP2-15676-img0002.jpg "Western Blot: Calpain 2 Antibody [NBP2-15676] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP2-15676] - Calpain 2 antibody detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15676-img0004.jpg)
![Western Blot: Calpain 2 Antibody [NBP2-15676] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Western-Blot-NBP2-15676-img0001.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP2-15676] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15676-img0003.jpg)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0005.jpg "Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0003.jpg)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0004.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Hela cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0001.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Jurkat cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0002.jpg)
![Knockout Validated: Calpain 2 Antibody [NBP2-92949] - Analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using Calpain 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Knockout-Validated-NBP2-92949-img0002.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Calpain 2 Antibody [NBP2-92949] - Analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using Calpain 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in")
![Western Blot: Calpain 2 Antibody (9Y7I1) [NBP3-16324] - Western blot analysis of extracts of various cell lines, using Calpain 2 Rabbit mAb (NBP3-16324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-9Y7I1-Western-Blot-NBP3-16324-img0001.jpg "Western Blot: Calpain 2 Antibody (9Y7I1) [NBP3-16324] - Western blot analysis of extracts of various cell lines, using Calpain 2 Rabbit mAb (NBP3-16324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![SDS-Page: Recombinant Human Calpain 2 Protein [H00000824-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Calpain-2-Protein-SDS-Page-H00000824-P01-img0002.jpg "SDS-Page: Recombinant Human Calpain 2 Protein [H00000824-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Rat Calpain 2 ELISA Kit (Colorimetric) [NBP2-74981] - Samples were spiked with high concentrations of Rat Calpain 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-74981_rat-calpain-2-elisa-kit-colorimetric-132202416232358.jpg "ELISA: Rat Calpain 2 ELISA Kit (Colorimetric) [NBP2-74981] - Samples were spiked with high concentrations of Rat Calpain 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Calpain 2 ELISA Kit (Colorimetric) [NBP2-74981] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-74981_rat-calpain-2-elisa-kit-colorimetric-432024855029.jpg)
![Western Blot: Calpain 2 Overexpression Lysate (Adult Normal) [NBL1-08680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08680-img0002.jpg "Western Blot: Calpain 2 Overexpression Lysate (Adult Normal) [NBL1-08680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calpain 2.")
![Western Blot: Calpain 2 Antibody (17E9) [NBP3-26467] - Positive Western Blot detected in: U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate. All lanes: Calpain 2 Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 80, 72 kDa Observed band size: 72-95 kDa](http://images.novusbio.com/fullsize/nbp3-26467_rabbit-calpain-2-mab-17e9-282202411485813.jpg "Western Blot: Calpain 2 Antibody (17E9) [NBP3-26467] - Positive Western Blot detected in: U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate. All lanes: Calpain 2 Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 80, 72 kDa Observed band size: 72-95 kDa")