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BLIMP1/PRDM1 Antibody

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Biological Strategies: Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180] - Analysis of BLIMP-1 using BLIMP-1 polyclonal antibody in cattle skin extracts from 5 different animals. Protein expression of increased ...read more

Product Details

Summary
Reactivity Hu, Bv, Mu, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

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BLIMP1/PRDM1 Antibody Summary

Immunogen
Amino acids 692-719 of human BLIMP-1 (PRDM1) were used as the immunogen for this antibody.
Marker
Plasma Cell Marker
Specificity
It is 100% homologous in isoforms 1 and 2 of human BLIMP-1 (PRDM1).
Predicted Species
Mouse (94%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRDM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Publications
Read Publication using
NBP2-27180 in the following applications:

  • WB
    1 publication

Reactivity Notes

The BLIMP-1 (PRDM1) amino acid sequence used as immunogen for this antibody is 85% homologous in mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml crude whole Rabbit sera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for BLIMP1/PRDM1 Antibody

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP1
  • BLIMP-1
  • BLIMP1MGC118925
  • blmp-1
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

An 88kDa transcription repressor protein is a human homolog of mouse Blimp-1. It belongs to the family of PRDM family and contains a PR domain and 5 kruppel Like-zinc finger motifs at the C-terminal region. BLIMP-1 plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes. It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter. BLIMP-1 silences IFNi -gene expression by binding specifically to PRDI of the IFNi gene promoter. It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells. In myeloma cells, PRDI-BF1i , a truncated isoform of BLIMP-1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BLIMP1/PRDM1 Antibody (NBP2-27180)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (NBP2-27180). (Showing 1 - 1 of 1 FAQ).

  1. I would like to use this antibody for western blotting. Is it recommended for WB test?
    • This antibody has been validated for Western blotting with samples from human and bovine, and I can confirm that the immunogen sequence for NBP2-27180 is 100% homologous in human, chimp, dog and bovine, 94% homologous in mouse, and 88% homologous in chicken, zebrafish and rat. As such, the antibody would be expected to react with zebrafish Blimp-1, although we do not currently have any testing data from zebrafish that I can share with you.

Secondary Antibodies

 

Isotype Controls

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Blogs on BLIMP1/PRDM1.

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Bioinformatics

Gene Symbol PRDM1