Novus Biologicals products are now on bio-techne.com

BLIMP1/PRDM1 Antibody

Images

 
Biological Strategies: Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180] - Analysis of BLIMP-1 using BLIMP-1 polyclonal antibody in cattle skin extracts from 5 different animals. Protein expression of increased ...read more

Product Details

Summary
Reactivity Hu, Bv, Mu, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

BLIMP1/PRDM1 Antibody Summary

Immunogen
Amino acids 692-719 of human BLIMP-1 (PRDM1) were used as the immunogen for this antibody.
Marker
Plasma Cell Marker
Specificity
It is 100% homologous in isoforms 1 and 2 of human BLIMP-1 (PRDM1).
Predicted Species
Mouse (94%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRDM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Publications
Read Publication using
NBP2-27180 in the following applications:

  • WB
    1 publication

Reactivity Notes

The BLIMP-1 (PRDM1) amino acid sequence used as immunogen for this antibody is 85% homologous in mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml crude whole Rabbit sera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for BLIMP1/PRDM1 Antibody

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP1
  • BLIMP-1
  • BLIMP1MGC118925
  • blmp-1
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

An 88kDa transcription repressor protein is a human homolog of mouse Blimp-1. It belongs to the family of PRDM family and contains a PR domain and 5 kruppel Like-zinc finger motifs at the C-terminal region. BLIMP-1 plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes. It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter. BLIMP-1 silences IFNi -gene expression by binding specifically to PRDI of the IFNi gene promoter. It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells. In myeloma cells, PRDI-BF1i , a truncated isoform of BLIMP-1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-59786
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB3487
Species: Hu, Mu
Applications: Flow, ICC, IHC, mIF, Simple Western, WB
NBP1-77681
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
AF2780
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
AF5525
Species: Hu
Applications: IHC, WB
6507-IL/CF
Species: Hu
Applications: BA
202-IL
Species: Hu
Applications: BA
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NBP1-89644
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF594
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
AF632
Species: Hu
Applications: AgAct, Simple Western, WB
7268-CT
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DY417
Species: Mu
Applications: ELISA
NBP1-91011
Species: Hu
Applications: IHC, IHC-Fr, IHC-P, WB
AF114
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
M6000B
Species: Mu
Applications: ELISA
M5000
Species: Mu
Applications: ELISA
NBP2-27180
Species: Hu, Bv, Mu, Ca, Pm
Applications: WB
Supplier Logo

Publications for BLIMP1/PRDM1 Antibody (NBP2-27180)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Bovine
(1)
All Species

Reviews for BLIMP1/PRDM1 Antibody (NBP2-27180) (0)

There are no reviews for BLIMP1/PRDM1 Antibody (NBP2-27180). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (NBP2-27180). (Showing 1 - 1 of 1 FAQ).

  1. I would like to use this antibody for western blotting. Is it recommended for WB test?
    • This antibody has been validated for Western blotting with samples from human and bovine, and I can confirm that the immunogen sequence for NBP2-27180 is 100% homologous in human, chimp, dog and bovine, 94% homologous in mouse, and 88% homologous in chicken, zebrafish and rat. As such, the antibody would be expected to react with zebrafish Blimp-1, although we do not currently have any testing data from zebrafish that I can share with you.

Secondary Antibodies

 

Isotype Controls

Additional BLIMP1/PRDM1 Products

Research Areas for BLIMP1/PRDM1 Antibody (NBP2-27180)

Find related products by research area.

Blogs on BLIMP1/PRDM1.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BLIMP1/PRDM1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PRDM1