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RIZ1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RIZ1 Antibody [NBP1-89644] - Staining of human cell line U-251MG shows positivity in nucleus and golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human gallbladder shows weak cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Analysis in human cerebral cortex and liver tissues using NBP1-89644 antibody. Corresponding PRDM2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human liver shows very weak positivity in hepatocytes.
Immunohistochemistry-Paraffin: RIZ1 Antibody [NBP1-89644] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RIZ1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PPPFQYHHRNPMGIGVTATNFTTHNIPQTFTTAIRCTKCGKGVDNMPELHKHILACASASDKKRYTPKKNPVPLKQTVQPKNGVVVLDNSGKNAFRRMGQPKRLNFSVELSKMSSNKLKLNALKKKNQLVQKAI
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRDM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RIZ1 Protein (NBP1-89644PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RIZ1 Antibody

  • EC 2.1.1.43
  • GATA-3 binding protein G3B
  • GATA-3-binding protein G3B
  • KMT8PR domain-containing protein 2
  • Lysine N-methyltransferase 8
  • MTB-ZFHUMHOXY1
  • PR domain containing 2, with ZNF domain
  • PR domain zinc finger protein 2
  • retinoblastoma protein-binding zinc finger protein
  • Retinoblastoma protein-interacting zinc finger protein
  • RIZ1
  • RIZ2
  • RIZMTE-binding protein
  • Zinc finger protein RIZ
  • zinc-finger DNA-binding protein

Background

RIZ1 (Retinoblastoma proteininteracting zinc finger), also known as PRDM2, is a nuclear protein containing 8 zinc finger motifs. RIZ1 contains a PR domain that may function as protein binding interface in the regulation of chromatin-mediated gene expression. RIZ 1 acts as a tumor suppressor, and RIZ 1 inactivation is common in many types of human cancers, including neuroendocrine, breast, liver, colon, skin, and lymphoid tumors. Similarly, RIZ1 over-expression in breast cancer cells caused cell cycle arrest in G2-M and apoptosis. RIZ1 is highly expressed in human retinoblastoma cells and at low levels in all other human cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional RIZ1 Products

Array NBP1-89644

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Bioinformatics

Gene Symbol PRDM2