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Syndecan-1/CD138 Antibody

 (13 Publications)
 (1 Review)
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Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL ...read more
Syndecan-1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Biotin
BAF2780
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Carrier Free
AF2780-SP
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Syndecan-1/CD138 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988
Specificity
Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SDC1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Syndecan-1/CD138 Antibody (Catalog # AF2780)
ELISA Detection:Human Syndecan-1/CD138 Biotinylated Antibody (Catalog # BAF2780)
Standard: Recombinant Human Syndecan-1/CD138 (Catalog # 2780-SD)
Reviewed Applications
Read 1 Review rated 5
using
AF2780 in the following applications:
Publications
Read Publications using
AF2780 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-1/CD138 Antibody

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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    Publications for Syndecan-1/CD138 Antibody (AF2780)(13)

    We have publications tested in 2 confirmed species: Human, Mouse.

    We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunodepletion, Immunoprecipitation, Western Blot.


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    Flow Cytometry
    (1)
    ICC
    (1)
    IHC
    (2)
    IHC-P
    (1)
    Immunodepletion
    (2)
    Immunoprecipitation
    (1)
    Western Blot
    (3)
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    Human
    (10)
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    (1)
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    Showing Publications 1 - 10 of 13. Show All 13 Publications.
    Publications using AF2780 Applications Species
    Jian Song, Tushar Deshpande, Xueli Zhang, Melanie-Jane Hannocks, Nils Lycke, Susanna L. Cardell et al. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation iScience 5/19/2023 [PMID: 37234087]
    MP Garrido, I Hurtado, M Valenzuela, R Salvatierr, A Hernández, M Vega, A Selman, AFG Quest, C Romero NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE2 Signaling Axis Cancers (Basel), 2019-12-07;11(12):. 2019-12-07 [PMID: 31817839] (Western Blot, Human) Western Blot Human
    Christopher S. Smillie, Moshe Biton, Jose Ordovas-Montanes, Keri M. Sullivan, Grace Burgin, Daniel B. Graham et al. Cellular and inter-cellular rewiring of the human colon during ulcerative colitis Cell 7/1/2019 [PMID: 31348891]
    Emmanuelle Fleurot, Caroline Goudin, Vincent Hanoux, Pierre-Jacques Bonnamy, Jérôme Levallet Estrogen receptor alpha regulates the expression of syndecan-1 in human breast carcinoma cells Endocrine-Related Cancer 6/1/2019 [PMID: 30978702]
    AM Hoffmann-V, SS Weigt, V Palchevski, E Volkmann, R Saggar, N Li, Ø Midtvedt, MB Lund, T Garen, MC Fishbein, A Ardehali, DJ Ross, T Ueland, P Aukrust, JP Lynch, RM Elashoff, Ø Molberg, JA Belperio Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis PLoS ONE, 2018-11-20;13(11):e0206545. 2018-11-20 [PMID: 30457999] (IHC-P, Human) IHC-P Human
    S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation Nat Commun, 2018-09-11;9(1):3686. 2018-09-11 [PMID: 30206230] (IHC, Human) IHC Human
    MA Qasaimeh, YC Wu, S Bose, A Menachery, S Talluri, G Gonzalez, M Fulciniti, JM Karp, RH Prabhala, R Karnik Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device Sci Rep, 2017-04-04;7(0):45681. 2017-04-04 [PMID: 28374831] (Immunoprecipitation, Human) Immunoprecipitation Human
    Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions. J Biol Chem, 2015-11-24;291(4):1652-63. 2015-11-24 [PMID: 26601950] (Flow Cytometry, Human) Flow Cytometry Human
    Stewart M, Ramani V, Sanderson R Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk. J Biol Chem, 2014-11-17;290(2):941-9. 2014-11-17 [PMID: 25404732] (Western Blot, Human, Mouse) Western Blot Human, Mouse
    Ramani V, Sanderson R Chemotherapy stimulates syndecan-1 shedding: a potentially negative effect of treatment that may promote tumor relapse. Matrix Biol, 2013-10-18;35(0):215-22. 2013-10-18 [PMID: 24145151] (Immunodepletion, Western Blot, Human) Immunodepletion, Western Blot Human
    Show All 13 Publications.

    Review for Syndecan-1/CD138 Antibody (AF2780) (1) 51

    Average Rating: 5
    (Based on 1 review)
    We have 1 review tested in 1 species: Human.

    Reviews using AF2780:
    Filter by Applications
    IF
    (1)
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    Human
    (1)
    All Species
    Images Ratings Applications Species Date Details
    Immunofluorescence Syndecan-1/CD138 AF2780
    Enlarge         		5
    reviewed by:
    Natalie Erdmann
    		 IF Human 03/13/2015
    View

    Summary

    ApplicationImmunofluorescence
    Sample TestedRPMI-8226 multiple myeloma cell line, and Daudi Burkitt lymphoma cell line
    SpeciesHuman

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    Immunofluorescence Syndecan-1/CD138 AF2780
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    		 Natalie Erdmann
    03/13/2015
    Application: IF
    Species: Human
    read this review

    Bioinformatics

    Gene Symbol SDC1
    Uniprot