![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, ZeSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437] |
| Localization | Major constituent of microtubules |
| Marker | Microtubule Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TUBB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells. |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/16250.jpg)
![Immunocytochemistry beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/beta-III_Tubulin_MAB1195_Immunocytochemistry_6556.jpg)
![Immunohistochemistry beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/betaIII_Tubulin_MAB1195_Immunohistochemistry_22757.jpg)
![Immunohistochemistry Galanin R2/GALR2 Antibody (616520) [Unconjugated]](https://images.novusbio.com/images2/Galanin_R2_MAB6544_Immunohistochemistry_10364.jpg)
![Flow Cytometry Galanin R2/GALR2 Antibody (616520) [Unconjugated]](https://images.novusbio.com/images2/Galanin_R2_MAB6544_Flow_Cytometry_23555.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Adeline Williams |
WB | Ae. aegypti | 05/22/2021 |
Summary
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reviewed by:
Verified Customer |
Simple Western | Mouse | 01/21/2016 |
Summary
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reviewed by:
Liyong Zhang |
WB | Mouse | 07/01/2014 |
Summary
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-(01-ml)_NB600-936_8221.bmp)
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reviewed by:
Bryan Tinsley |
ICC | Mouse | 06/12/2014 |
Summary
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reviewed by:
Bryan Tinsley |
WB | Human | 05/27/2014 |
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reviewed by:
Verified Customer |
IHC | Mouse | 01/13/2012 |
Summary
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reviewed by:
Verified Customer |
WB | Other | 11/09/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta Tubulin Antibody (NB600-936)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Adeline Williams 05/22/2021 |
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| Application: | WB | |
| Species: | Ae. aegypti |
| Verified Customer 01/21/2016 |
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| Application: | Simple Western | |
| Species: | Mouse |
| Liyong Zhang 07/01/2014 |
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| Application: | WB | |
| Species: | Mouse |
![Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg "Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg "Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000")
![Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg "Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0023.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].")
![Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0004.jpg "Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.")
![Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep29311), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0024.jpg "Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep29311), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0015.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0001.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0022.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0014.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].")
![Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0013.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].")
![Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0012.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].")
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_723.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_724.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/CXCL10_DIP100_ELISA_121.jpg)
![Immunohistochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunohistochemistry_10417.jpg)
![Immunocytochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunocytochemistry_11095.jpg)
![Western Blot Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Western_Blot_9601.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NB600-936_8571.jpg)