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beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

Images

 
Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse ...read more
beta -III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 ...read more
beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours ...read more
beta -III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 ...read more
beta -III Tubulin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 8 µg/mL for 1 ...read more
Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse ...read more
beta ‑III Tubulin was detected in immersion fixed paraffin-embedded sections of Human Brain Cerebellum using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) at ...read more
beta ‑III Tubulin was detected in immersion fixed SH‑SY5Y human neuroblastoma cells (Positive) & absent in HEL 92.1.7 human erythroleukemic cells (Negative) using Mouse Anti-Neuron-specific beta ...read more
HepG2 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
I alpha I maintains pluripotency and differentiation capacity in long-term culture.Immunofluorescence staining of H207 hES cell line for (a) stem cell markers Oct4 (green), Sox2 (green), Nanog (red) and SSEA-4 (green) ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
Representative immunocytochemistry demonstrating the expression of neuronal, synaptic, glutamatergic, GABAergic, and glial markers in cultures 21 days after plating. Neuronal cultures were immunostained against beta ...read more
Differentiation of murine skin stem cell precursors (SKP).The differentiation of SKPs was induced through the dissociation of cell aggregates into single cells followed by serum exposure and, as may be seen in the ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
Neuronal markers expressed by iPSC-NSCs after differentiation and maturation on PEG hydrogels.(a) beta III-tubulin (red), GAD 65/67 (green), and DAPI (nuclei, blue). Single channel grayscale images from (a) are shown ...read more
Differentiation of murine skin stem cell precursors (SKP).The differentiation of SKPs was induced through the dissociation of cell aggregates into single cells followed by serum exposure and, as may be seen in the ...read more
CTCL is associated with nerve innervations in mouse lymphoma.(A) Double immunostaining for CGRP and beta –tubulin III in the back skin with lymphoma from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
I alpha I maintains pluripotency and differentiation capacity in long-term culture.Immunofluorescence staining of H207 hES cell line for (a) stem cell markers Oct4 (green), Sox2 (green), Nanog (red) and SSEA-4 (green) ...read more
CTCL is associated with nerve innervations in mouse lymphoma.(A) Double immunostaining for CGRP and beta –tubulin III in the back skin with lymphoma from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, IHC, Flow, ICC/IF
Clone
TuJ-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific Summary

Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 0.5-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 9 Reviews rated 4.7
using
MAB1195 in the following applications:

Publications
Read Publications using
MAB1195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB1195
Species: All-Multi
Applications: WB, Simple Western, IHC, Flow, ICC/IF

Publications for beta-III Tubulin Antibody (MAB1195)(349)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Amphibian, Amphibian - Axolotl, Avian - Chicken, Avian - Quail, Axolotl, Axolotl - Ambystoma mexicanum, Chicken, Macaca mulatta (Rhesus Macaque), Porcine, Primate - Papio anubis (Olive Baboon), Salamander, Transgenic Mouse, Xenograft.

We have publications tested in 15 applications: Flow Cytometry, HSI Microscopy, ICC, ICC/IF, IF, IF/ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, Simple Western, Western Blot.


Filter By Application
Flow Cytometry
(12)
HSI Microscopy
(1)
ICC
(122)
ICC/IF
(1)
IF
(1)
IF/ICC
(1)
IHC
(48)
IHC-Fr
(21)
IHC-P
(9)
Immunocytochemistry
(10)
Immunohistochemistry
(5)
Immunoprecipitation
(1)
Neutralization
(1)
Simple Western
(2)
Western Blot
(26)
All Applications
Filter By Species
Human
(84)
Mouse
(94)
Rat
(36)
Amphibian
(1)
Amphibian - Axolotl
(1)
Avian - Chicken
(2)
Avian - Quail
(1)
Axolotl
(2)
Axolotl - Ambystoma mexicanum
(1)
Chicken
(4)
Macaca mulatta (Rhesus Macaque)
(1)
Porcine
(2)
Primate - Papio anubis (Olive Baboon)
(1)
Salamander
(1)
Transgenic Mouse
(5)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 349. Show All 349 Publications.
Publications using MAB1195 Applications Species
Kokotos, AC;Antoniazzi, AM;Unda, SR;Ko, MS;Park, D;Eliezer, D;Kaplitt, MG;De Camilli, P;Ryan, TA; Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits Science advances 2024-08-23 [PMID: 39167658] (Immunocytochemistry, Mouse) Immunocytochemistry Mouse
Cantor, EL;Shen, F;Jiang, G;Philips, S;Schneider, BP; Optimization of a human induced pluripotent stem cell-derived sensory neuron model for the in vitro evaluation of taxane-induced neurotoxicity Scientific reports 2024-08-17 [PMID: 39154055] (Immunocytochemistry, Human) Immunocytochemistry Human
Lewis, PA;Silajdži?, E;Smith, H;Bates, N;Smith, CA;Mancini, FE;Knight, D;Denning, C;Brison, DR;Kimber, SJ; A secreted proteomic footprint for stem cell pluripotency PloS one 2024-06-14 [PMID: 38875182] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Barron, A;Barrett, L;Tuulari, JJ;Karlsson, L;Karlsson, H;McCarthy, CM;O'Keeffe, GW; sFlt-1 Impairs Neurite Growth and Neuronal Differentiation in SH-SY5Y Cells and Human Neurons Bioscience reports 2024-05-03 [PMID: 38700092] (Immunocytochemistry, Human) Immunocytochemistry Human
Brown, JA;Faley, SL;Judge, M;Ward, P;Ihrie, RA;Carson, R;Armstrong, L;Sahin, M;Wikswo, JP;Ess, KC;Neely, MD; Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit Journal of neurodevelopmental disorders 2024-05-23 [PMID: 38783199] (Immunocytochemistry, Human) Immunocytochemistry Human
Echeverria, CV;Leathers, TA;Rogers, CD; Effectiveness of fixation methods for wholemount immunohistochemistry across cellular compartments in chick embryos bioRxiv : the preprint server for biology 2024-03-25 [PMID: 38585750] (Immunohistochemistry, Chicken) Immunohistochemistry Chicken
Jo-Watanabe, A;Inaba, T;Osada, T;Hashimoto, R;Nishizawa, T;Okuno, T;Ihara, S;Touhara, K;Hattori, N;Oh-Hora, M;Nureki, O;Yokomizo, T; Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury Nature communications 2024-02-27 [PMID: 38413581] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Ladaika, CA;Ghobashi, AH;Boulton, WC;Miller, SA;O'Hagan, HM; Single-cell multi-omics reveals insights into differentiation of rare cell types in mucinous colorectal cancer bioRxiv : the preprint server for biology 2024-02-05 [PMID: 38370733] (Immunocytochemistry, Human) Immunocytochemistry Human
Majumder, J;Torr, EE;Aisenbrey, EA;Lebakken, CS;Favreau, PF;Richards, WD;Yin, Y;Chang, Q;Murphy, WL; Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels Journal of tissue engineering 2024-02-14 [PMID: 38361535] (Immunocytochemistry, Human) Immunocytochemistry Human
Show All 349 Publications.

Reviews for beta-III Tubulin Antibody (MAB1195) (9) 4.79

Average Rating: 4.7
(Based on 9 reviews)
We have 9 reviews tested in 4 species: Human, Mouse, Feline, Other.

Reviews using MAB1195:
Filter by Applications
ICC
(2)
Immunofluorescence Acetone fixed
(1)
IHC-Fr
(2)
WB
(1)
IF
(3)
All Applications
Filter by Species
Human
(3)
Mouse
(4)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Patricia Carulla Martí
ICC Human 05/25/2023
View

Summary

ApplicationImmunocytochemistry
Sample TestedNeuroblastoma cells SH-SY5Y differentiated into neurons
SpeciesHuman
LotHGQ0421071
Other beta-III Tubulin MAB1195
Enlarge
4
reviewed by:
Jan Martinek
Immunofluorescence Acetone fixed Mouse 01/17/2019
View

Summary

ApplicationOther
Sample TestedAdult mouse brain tissue
SpeciesMouse
LotHGQ0218071
  5
reviewed by:
Verified Customer
IHC-Fr Feline 11/02/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult brain
SpeciesFeline
LotHGQ0217041
  5
reviewed by:
Verified Customer
WB Mouse 02/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23158765
SpeciesMouse
  5
reviewed by:
Verified Customer
IF Mouse 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19409493
SpeciesMouse
  5
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22985741
SpeciesHuman
  5
reviewed by:
Verified Customer
ICC Mouse 02/04/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 20722977
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-Fr Other 02/04/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 22120963
SpeciesOther
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-III Tubulin Antibody (MAB1195) (0)

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Secondary Antibodies

 

Isotype Controls

Additional beta-III Tubulin Products

Blogs on beta-III Tubulin.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Recent Reviews

4.7
5
6
4
3
3
0
2
0
1
0

Patricia Carulla Martí
05/25/2023
Application: ICC
Species: Human

Jan Martinek
01/17/2019
Application: Immunofluorescence Acetone fixed
Species: Mouse

Verified Customer
11/02/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol TUBB3