alpha-Smooth Muscle Actin Antibody

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 6 applications: IF/IHC, IHC, IHC-P, Immunohistochemistry, In Vivo, WB.

Showing Publications 1 - 10 of 10.

Publications using NBP1-30894	Applications	Species
Takada, H;Sasagawa, Y;Yoshimura, M;Tanaka, K;Iwayama, Y;Hayashi, T;Isomura-Matoba, A;Nikaido, I;Kurisaki, A; Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis Nature communications 2023-06-29 [PMID: 37386010] (In Vivo)	In Vivo
Gui T, Chen Q, Li J et al. Astragaloside IV alleviates 1-deoxysphinganine-induced mitochondrial dysfunction during the progression of chronic kidney disease through p62-Nrf2 antioxidant pathway Frontiers in Pharmacology 2023-03-24 [PMID: 37033627] (Immunohistochemistry)	Immunohistochemistry
Hong E, Barczak W, Park S et al. Combination treatment of T1-44, a PRMT5 inhibitor with Vactosertib, an inhibitor of TGF-? signaling, inhibits invasion and prolongs survival in a mouse model of pancreatic tumors Cell death & disease 2023-02-10 [PMID: 36765032] (IHC, Mouse)	IHC	Mouse
Wu M, Matar DY, Yu Z et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing Pharmaceutics 2022-10-06 [PMID: 36297560] (IHC-P, Mouse)	IHC-P	Mouse
Wu M, Yu Z, Matar DY et al. Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model Advances in wound care 2022-03-16 [PMID: 35293255] (IF/IHC, Mouse)	IF/IHC	Mouse
Xie J, Yuan S, Peng L et al. Antitumor immunity targeting fibroblast activation protein‑ alpha in a mouse Lewis lung carcinoma model Oncol Lett 2020-05-18 [PMID: 32566014] (WB, Mouse)	WB	Mouse
Miyadera K, Conatser L, Llanga TA et al. Intrastromal Gene Therapy Prevents and Reverses Advanced Corneal Clouding in a Canine Model of Mucopolysaccharidosis I Mol. Ther. 2020-04-11 [PMID: 32330426] (IF/IHC, Canine)	IF/IHC	Canine
Hong E, Park S, Ooshima A et al. Inhibition of TGF-beta signalling in combination with nal-IRI plus 5-Fluorouracil/Leucovorin suppresses invasion and prolongs survival in pancreatic tumour mouse models Sci Rep 2020-02-19 [PMID: 32076068] (IF/IHC, Mouse)	IF/IHC	Mouse
Gai Z, Visentin M, Gui T et al. The effects of farnesoid X receptor activation on arachidonic acid metabolism, NF-kB signaling and hepatic inflammation Mol. Pharmacol. 2018-05-09 [PMID: 29743187] (IF/IHC, Human)	IF/IHC	Human
Gai Z, Chu L, Xu Z et al. Farnesoid X receptor activation protects the kidney from ischemia-reperfusion damage Sci Rep 2017-08-29 [PMID: 28852062] (IHC-P, Mouse)	IHC-P	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	01/07/2021	View Summary Application Immunohistochemistry-Frozen Sample Tested E11.5 mouse embryo fixed in 4% PFA Species Mouse Lot 43040 Comments Comments Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 1:100 Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000
Enlarge	5 reviewed by: Julie Kiland	ICC	Feline	05/18/2017	View Summary Application Immunocytochemistry Sample Tested Vascular Smooth Muscle Cells Species Feline Lot 42046

Array NBP1-30894

• alpha-Smooth Muscle Actin Antibodies
• alpha-Smooth Muscle Actin Antibody Pair
• alpha-Smooth Muscle Actin ELISA Kits
• alpha-Smooth Muscle Actin Lysates
• alpha-Smooth Muscle Actin Proteins