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alpha Adducin Antibody

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Western Blot: alpha Adducin Antibody [NBP2-15359] - A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE alpha Adducin antibody ...read more
Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-15359] - Paraffin-embedded SG xenograft, using antibody at 1:100 dilution.
Western Blot: alpha Adducin Antibody [NBP2-15359] - Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Ze, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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alpha Adducin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.
Localization
Cytoplasm; cytoskeleton; Cell membrane
Predicted Species
Rat (98%), Chicken (98%), Xenopus (93%), Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADD1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%), Zebrafish (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for alpha Adducin Antibody

  • ADDA
  • adducin 1 (alpha)
  • alpha-adducin
  • erythrocyte adducin alpha subunit
  • Erythrocyte adducin subunit alpha
  • MGC3339
  • MGC44427

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADD1
Uniprot