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TYRP1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Analysis in human skin and cerebral cortex tissues. Corresponding TYRP1 RNA-seq data are presented for the same tissues.
Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in control (vector only transfected HEK293T lysate) and TYRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TYRP1 Antibody [NBP1-88370] - Staining of human cell line SK-MEL-30 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human rectum shows moderate to strong cytoplasmic positivity in a subset of glandular cells and cells in stroma.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

TYRP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VCDICTDDLMGSRSNFDSTLISPNSVFSQWRVVCDSLEDYDTLGTLCNSTEDGPIRRNPAGNVARPMVQRLPEPQDVAQCLEVGLFDTPPFYSNSTNSFRNTVEGYSDPTGKYDPAVRSLH
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TYRP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TYRP1 Protein (NBP1-88370PEP)
Publications
Read Publications using
NBP1-88370 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (Shridhar et al).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TYRP1 Antibody

  • b-PROTEIN
  • CAS25,6-dihydroxyindole-2-carboxylic acid oxidase
  • Catalase B
  • CATB
  • EC 1.14.18
  • EC 1.14.18.-
  • EC 1.14.18.1
  • Glycoprotein 75
  • GP75DHICA oxidase
  • Melanoma antigen gp75
  • TRP-1
  • TRPTYRP
  • tyrosinase-related protein 1TRP1
  • TYRRP

Background

TYRP1 encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TYRP1 Antibody (NBP1-88370)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP1-88370 Applications Species
Shridhar N Novel vaccination strategies for CD4+ T cell immunotherapy of melanoma Thesis (WB, Mouse) WB Mouse
Shridhar N Novel vaccination strategies for CD4+ T cell immunotherapy of melanoma Thesis (ICC/IF, Mouse) ICC/IF Mouse
Li MY, Flora P, Pu H Et al. UV-induced reduction in Polycomb repression promotes epidermal pigmentation Developmental cell 2021-08-30 [PMID: 34473941]
Mengoni M, Braun AD, Gaffal E, Tuting T C9orf72 in myeloid cells suppresses STING-induced inflammation Int. J. Cancer 2020-08-13 [PMID: 32790916] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TYRP1 Antibody (NBP1-88370) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on TYRP1.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

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Bioinformatics

Gene Symbol TYRP1