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alpha-Actinin 1 Antibody (OTI7A4) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clone
OTI7A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405

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alpha-Actinin 1 Antibody (OTI7A4) [Alexa Fluor® 405] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 650-893 of human alpha-Actinin 1 (NP_001093) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for alpha-Actinin 1 Antibody (OTI7A4) [Alexa Fluor® 405]

  • actinin 1 smooth muscle
  • actinin, alpha 1
  • ACTN1
  • alpha Actinin 1
  • alphaActinin 1
  • alpha-Actinin 1
  • Alpha-actinin cytoskeletal isoform
  • alpha-actinin-1
  • BDPLT15
  • F-actin cross-linking protein
  • FLJ40884
  • FLJ54432
  • Non-muscle alpha-actinin-1

Background

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACTN1