![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0002.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clone | OTI7A4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 102.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Actinin 1 Antibody (NBP2-70078)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACTN1 |
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0004.jpg "Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0008.jpg "Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.")
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Alpha Actinin 1 antibody (7A4) at 1:2000 + recombinant human Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0001.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Alpha Actinin 1 antibody (7A4) at 1:2000 + recombinant human Alpha Actinin 1.")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0003.jpg "Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0005.jpg "Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0006.jpg "Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0007.jpg "Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0009.jpg "Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.")
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