Do you have any 100 coda or bigger controls?

Name: Vinculin Antibody (728526) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB6896
Rating: 4.3 (3 reviews)

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Accepted Answer

I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Simple Western, IHC
Clone	728526
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB6896-SP

Immunogen	E. coli-derived recombinant human Vinculin Lys1020-Gln1134 Accession # P18206
Specificity	Detects human Vinculin in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	VCL
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Sterile PBS to a final concentration of 0.5 mg/mL.

Vinculin Antibody (728526) [Unconjugated]

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Publications for Vinculin Antibody (MAB6896)(19)

Reviews for Vinculin Antibody (MAB6896) (3) 4.33

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Publications using MAB6896	Applications	Species
Park M, Woo H N et al. A Single Injection of rAAV-shmTOR in Peripheral Nerve Persistently Attenuates Nerve Injury-Induced Mechanical Allodynia. Int J Mol Sci 2024-11-02 [PMID: 37958901] (Simple Western, Rat) Details: 1:100	Simple Western	Rat
Sproule, TJ;Wilpan, RY;Low, BE;Silva, KA;Reyon, D;Joung, JK;Wiles, MV;Roopenian, DC;Sundberg, JP; Functional analysis of Collagen 17a1: A genetic modifier of junctional epidermolysis bullosa in mice PloS one 2023-10-05 [PMID: 37796769] (Western Blot, Mouse, Transgenic Mouse)	Western Blot	Mouse, Transgenic Mouse
Corina E. Antal, Tae Gyu Oh, Stefan Aigner, En-Ching Luo, Brian A. Yee, Tania Campos et al. A super-enhancer-regulated RNA-binding protein cascade drives pancreatic cancer Nat Commun 9/6/2023 [PMID: 37673892]
L Wang, B Lin, M Zhai, W Cui, L Hull, A Zizzo, X Li, JG Kiang, M Xiao Deteriorative Effects of Radiation Injury Combined with Skin Wounding in a Mouse Model Toxics, 2022-12-14;10(12):. 2022-12-14 [PMID: 36548618] (Western Blot, Mouse)	Western Blot	Mouse
S Mohammadho, LC Ho, L Fang, J Xu, F Medina-Bol Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells International Journal of Molecular Sciences, 2022-01-20;23(3):. 2022-01-20 [PMID: 35163062] (Human)		Human
A Baradaran-, CC Bauer, IB Pickles, S Hosseini-F, AD Balgi, K Choi, DM Linley, DJ Beech, M Roberge, RS Bon Non-selective TRPC channel inhibition and suppression of aminoglycoside-induced premature termination codon readthrough by the small molecule AC1903 The Journal of Biological Chemistry, 2022-01-06;298(2):101546. 2022-01-06 [PMID: 34999117] (Simple Western, Human)	Simple Western	Human
Maysaa El Sayed Zaki, Dina Elhammady, Mona Foda Salama, Mostafa Abdelsalam, Asmaa Osama Bakr Osman Study of Antibodies to Cytolethal Distending Toxin B (CdtB) and Antibodies to Vinculin in Patients with Irritable Bowel Syndrome F1000Research 10/15/2021 [PMID: 34754418]
GR Lee, D Gallo, RW Alves de S, S Tiwari-Hec, E Csizmadia, JD Harbison, S Shankar, V Banner-Goo, MB Yaffe, MS Longhi, CJ Hauser, LE Otterbein Trauma-induced heme release increases susceptibility to bacterial infection JCI Insight, 2021-10-22;0(0):. 2021-10-22 [PMID: 34520397] (Western Blot, Mouse)	Western Blot	Mouse
Tabebordbar M, Lagerborg KA, Stanton A et al. Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species Cell 9/16/2021 [PMID: 34506722]
S Hosseini-F, A Baradaran-, C Zimmerman, K Choi, S Flibotte, M Roberge Small molecule Y-320 stimulates ribosome biogenesis, protein synthesis, and aminoglycoside-induced premature termination codon readthrough PloS Biology, 2021-05-03;19(5):e3001221. 2021-05-03 [PMID: 33939688] (Western Blot, Human)	Western Blot	Human
NN Madigan, MJ Polzin, G Cui, T Liewluck, MH Alsharabat, CJ Klein, AJ Windebank, G Mer, M Milone Filamentous tangles with nemaline rods in MYH2 myopathy: a novel phenotype Acta neuropathologica communications, 2021-04-29;9(1):79. 2021-04-29 [PMID: 33926564] (Western Blot, Human)	Western Blot	Human
A Baradaran-, AD Balgi, S Hosseini-F, K Choi, C Has, M Roberge Effect of small molecule eRF3 degraders on premature termination codon readthrough Nucleic Acids Research, 2021-04-19;0(0):. 2021-04-19 [PMID: 33764477] (Western Blot, Human)	Western Blot	Human
C Höring, U Seibel, K Tropmann, L Grätz, D Mönnich, S Pitzl, G Bernhardt, S Pockes, A Strasser A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes Int J Mol Sci, 2020-11-10;21(22):. 2020-11-10 [PMID: 33182741] (Western Blot, Human)	Western Blot	Human
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Western Blot, Human)	Western Blot	Human
Liu, G;Mateer, SW;Hsu, A;Goggins, BJ;Tay, H;Mathe, A;Fan, K;Neal, R;Bruce, J;Burns, G;Minahan, K;Maltby, S;Fricker, M;Foster, PS;Wark, PAB;Hansbro, PM;Keely, S; Platelet activating factor receptor regulates colitis-induced pulmonary inflammation through the NLRP3 inflammasome Mucosal Immunol 4/11/2019 [PMID: 30976089]
A Schramm, P Mueller-Th, T Littmann, M Harloff, T Ozawa, J Schlossman Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies Int J Mol Sci, 2018-04-12;19(4):. 2018-04-12 [PMID: 29649180] (Western Blot, Mouse)	Western Blot	Mouse
A Baradaran-, J Niesser, AD Balgi, K Choi, C Zimmerman, AP South, HJ Anderson, NC Strynadka, MB Bally, M Roberge Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity Proc. Natl. Acad. Sci. U.S.A, 2017-03-13;0(0):. 2017-03-13 [PMID: 28289221] (Western Blot Control, Mouse)	Western Blot Control	Mouse
Alireza Baradaran-, Aruna D Balgi, Carla Zimmerman, Kunho Choi, Fahimeh S Shidmooss, Jason S Tan, Célia Bergeaud, Alexandra Krause, Stéphane Flibotte, Yoko Shimizu, Hilary J Anderson, Vincent Mouly, Eric Jan, Tom Pfeifer, James B Jaquith, Michel Roberge Novel small molecules potentiate premature termination codon readthrough by aminoglycosides. Nucleic Acids Research, 2016-07-12;0(0):1362-4962. 2016-07-12 [PMID: 27407112] (Western Blot, Human)	Western Blot	Human
Naresh C Bal Increased reliance on muscle based thermogenesis upon acute minimization of brown adipose tissue function J Biol Chem, 2016-06-13;0(0):. 2016-06-13 [PMID: 27298322] (Western Blot, Mouse)	Western Blot	Mouse
Show All 19 Publications. Collapse Publications.

	Krishna Yalla 08/27/2020
Application:	Simple Western
Species:	Human

	Asimina Kerimi 09/23/2015
Application:	Simple Western
Species:	Mouse

	Verified Customer 10/16/2015
Application:	WB
Species:	Human

Gene Symbol	VCL
Uniprot	P18206()