AIF-1/Iba1 Antibody

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Monkey.

We have publications tested in 8 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 30. Show All 30 Publications. Collapse Publications.

Publications using NBP2-19019	Applications	Species
Tylek K, Trojan E, Le?kiewicz M et al. Microglia Depletion Attenuates the Pro-Resolving Activity of the Formyl Peptide Receptor 2 Agonist AMS21 Related to Inhibition of Inflammasome NLRP3 Signalling Pathway: A Study of Organotypic Hippocampal Cultures Cells 2023-11-03 [PMID: 37947648] (WB, Rat)	WB	Rat
Tsujikawa S, DeMeulenaere KE, Centeno MV et al. Regulation of neuropathic pain by microglial Orai1 channels Science Advances 2023-01-27 [PMID: 36706180] (Block/Neutralize)	Block/Neutralize
Zhou C, Nie S, Wang Z Et al. Fisetin has Inhibitory Effects on the TLR 4/NF- kappaB-Mediated Inflammatory Pathway After Traumatic Brain Injury in Mice: A Potential Neuroprotective Role Research Square 2021-08-11 (ICC/IF, Mouse)	ICC/IF	Mouse
Williams EP, Xue Y, Lee J et al. Deep spatial profiling of Venezuelan equine encephalitis virus reveals increased genetic diversity amidst neuroinflammation and cell death during brain infection Journal of Virology 2023-08-31 [PMID: 37560924]
Merlo S, Caruso GI, Korde DS et al. Melatonin Activates Anti-Inflammatory Features in Microglia in a Multicellular Context: Evidence from Organotypic Brain Slices and HMC3 Cells Biomolecules 2023-02-16 [PMID: 36830742] (WB, IHC, Mouse) Details: Dilution used in IHC 1:700	WB, IHC	Mouse
Ciechanowska A, Rojewska E, Piotrowska A et al. New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies Frontiers in immunology 2022-12-22 [PMID: 36618360] (WB, Mouse)	WB	Mouse
Guo L, Reed K, Carter A et al. Sleep-Disturbance-Induced Microglial Activation Involves CRH-Mediated Galectin 3 and Autophagy Dysregulation Cells 2022-12-30 [PMID: 36611953] (WB, Mouse)	WB	Mouse
Pawlik K, Ciapa?a K, Ciechanowska A et al. Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain Cells 2022-12-26 [PMID: 36611891]
Henriques A, Rouvi�re L, Giorla E et al. Alpha-Synuclein: The Spark That Flames Dopaminergic Neurons, In Vitro and In Vivo Evidence International Journal of Molecular Sciences 2022-08-30 [PMID: 36077253] (Immunohistochemistry)	Immunohistochemistry
Abhishek T, Shih-Ying W, Sambad S et al. Exosomal miR-4466 from nicotine-activated neutrophils promotes tumor cell stemness and metabolism in lung cancer metastasis Oncogene. 2022-04-23 [PMID: 35461327] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Xiang X, Tang X, Yu Y et al. Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia Journal of neuroinflammation 2022-04-12 [PMID: 35413913] (IF/IHC, Mouse)	IF/IHC	Mouse
Ma, N;Li, G;Fu, X; Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia Brain and behavior [PMID: 35263513]
Cheng Y, Kim WK, Wellman LL Et al. Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner Life (Basel, Switzerland) 2021-10-16 [PMID: 34685469] (Mouse)		Mouse
Rimal S, Li Y, Vartak R Et al. Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease Acta neuropathologica communications 2021-10-18 [PMID: 34663454] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Hong J, Adam RJ, Gao L et al. Macrophage activation in stellate ganglia contributes to lung injury-induced arrhythmogenesis in male rats Acta physiologica (Oxford, England) 2021-04-05 [PMID: 33817984]
Katja Niesel, Michael Schulz, Julian Anthes, Tijna Alekseeva, Jadranka Macas, Anna Salamero‐Boix, Aylin Möckl, Timm Oberwahrenbrock, Marco Lolies, Stefan Stein, Karl H Plate, Yvonne Reiss, Franz Rödel, Lisa Sevenich The immune suppressive microenvironment affects efficacy of radio‐immunotherapy in brain metastasis EMBO Molecular Medicine 2021-03-23 [PMID: 33755340]
Chamera K, Trojan E, Kotarska K et al. Role of Polyinosinic:Polycytidylic Acid-Induced Maternal Immune Activation and Subsequent Immune Challenge in the Behaviour and Microglial Cell Trajectory in Adult Offspring: A Study of the Neurodevelopmental Model of Schizophrenia International journal of molecular sciences 2021-02-04 [PMID: 33557113] (WB, Rat)	WB	Rat
Kim J, Suh YH, Chang KA Interleukin-17 induced by cumulative mild stress promoted depression-like behaviors in young adult mice Molecular brain 2021-01-13 [PMID: 33441182] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Williams AM, Manouchehri N, Erskine E et al. Cardio-centric hemodynamic management improves spinal cord oxygenation and mitigates hemorrhage in acute spinal cord injury Nat Commun 2020-10-16 [PMID: 33060602]
Liu L, Chen M, Lin K et al. Inhibiting Caspase-12 Mediated Inflammasome Activation protects against Oxygen-Glucose Deprivation Injury in Primary Astrocytes Int. J. Med. Sci. 2020-07-24 [PMID: 32788872] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Chamera K, Szuster-Gluszczak M, Trojan E, Basta-Kaim A Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200-CD200R and CX3CL1-CX3CR1 Systems Cells 2020-07-12 [PMID: 32664639] (WB, Rat)	WB	Rat
Hughes FM, Hirshman NA, Malick HA et al. A possible mechanism underlying mood disorders associated with LUTS: Chronic bladder outlet obstruction causes NLRP3-dependent inflammation in the hippocampus and depressive behavior in rats Neurourol. Urodyn. 2020-06-29 [PMID: 32602164]
Merlo S, Luaces JP, Spampinato SF et al. SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence Biomolecules 2020-02-27 [PMID: 32120833] (IF/IHC, IHC-P, Rat)	IF/IHC, IHC-P	Rat
Hirshman NA, Hughes FM, Jin H, et al. Cyclophosphamide-Induced Cystitis Results in NLRP3-Mediated Inflammation in the Hippocampus and Symptoms of Depression in Rats Am. J. Physiol. Renal Physiol. 2019-12-23 [PMID: 31869244] (IF/IHC, Rat)	IF/IHC	Rat
Xu G, Yamamoto N, Nojima T, et al. Intravenously infusing the secretome of adipose-derived mesenchymal stem cells ameliorates neuroinflammation and neurological functioning after traumatic brain injury Stem Cells Dev. 2019-12-13 [PMID: 31830866]
Han TU, Root J, Reyes LD et al. Human GNPTAB stuttering mutations engineered into mice cause vocalization deficits and astrocyte pathology in the corpus callosum Proc. Natl. Acad. Sci. U.S.A. 2019-08-27 [PMID: 31405983] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Merlo S, Spampinato SF, Beneventano M, Sortino MA. The contribution of microglia to early synaptic compensatory responses that precede B-amyloid-induced neuronal death Sci Rep 2018-05-08 [PMID: 29740062] (Human)		Human
Yang H, Fu Y, Liu X et al. Role of the sigma-1 receptor chaperone in rod and cone photoreceptor degenerations in a mouse model of retinitis pigmentosa Mol Neurodegener 2017-09-19 [PMID: 28927431] (WB, Mouse)	WB	Mouse
Madi A, Sheinin R, Salomon K et al. interFLOW: maximum flow framework for the identification of factors mediating the signaling convergence of multiple receptors Research Square 2023-10-31 (IHC, Mouse)	IHC	Mouse
Gurung S, Reuter N, Preno A et al. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon PLoS Pathog. 2019-01-01 [PMID: 30657788] (IHC-Fr, Monkey)	IHC-Fr	Monkey
Show All 30 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	1 reviewed by: Casey Nestor	IHC-Fr	Sheep	03/13/2020	View Summary Application Immunohistochemistry-Frozen Sample Tested Brain (hypothalamus) tissue Species Sheep Lot 43678 Comments Comments *Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody: To test, we used free-floating sheep hypothalamic brain sections from two animals. We did dilutions of 1:100 to 1:1000 and did not see any staining in our tissue. We also employed an antigen retrieval step in one of our runs, and it did not yield any different results.
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	12/18/2017	View Summary Application Western Blot Sample Tested Mouse forebrain lysate Species Mouse Lot 39476
	5 reviewed by: Verified Customer	ICC	Mouse	04/01/2017	View Summary Application Immunocytochemistry Sample Tested C57BL/6 mouse retina Species Mouse Lot N/A
Enlarge	4 reviewed by: Temugin Berta	ICC	Mouse	03/10/2017	View Summary Application Immunocytochemistry Sample Tested Dorsal root ganglia Species Mouse Lot 39476 Comments Comments Blocking and antibody solution: BSA1%/Triton 02% in PBS Dilution AIF1: 1/1000 Secondary antibody: Alexa 555 Counter-staining: Nissl

Array NBP2-19019

• AIF-1/Iba1 Antibodies
• AIF-1/Iba1 ELISA Kits
• AIF-1/Iba1 Lysate
• AIF-1/Iba1 Proteins