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ADAMTS3 Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF5465-SP
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ADAMTS3 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS3
Ser19-Cys712
Accession # O15072
Specificity
Detects human ADAMTS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 3% cross-reactivity with recombinant human (rh) ADAMTS4 and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS5, rhADAMTS13, and rhADAMTSL1.2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ADAMTS3
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS3 Antibody [Unconjugated]

  • A disintegrin and metalloproteinase with thrombospondin motifs 3
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 3
  • ADAM metallopeptidase with thrombospondin type 1 motif, 3
  • ADAM-TS 3
  • ADAMTS3
  • ADAM-TS3
  • ADAMTS-3
  • ADAMTS-4
  • EC 3.4.24
  • EC 3.4.24.-
  • EC 3.4.24.14
  • KIAA0366PC II-NP
  • PC II-NP
  • Procollagen II amino propeptide-processing enzyme
  • Procollagen II N-proteinase
  • zinc metalloendopeptidase

Background

ADAMTS3 (A disintegrin and metalloprotease with thrombospondin motifs 3; also PC II-NP) is a 140-150 kDa member of the ADAMTS family of Zn metalloproteases. It is expressed by osteoblasts, chrondrocytes, myoepithelium and fibroblasts, and participates in collagen maturation. Together with ADAMTS2 and TS14, ADAMTS3 is known to process the N-terminus of procollagen. In particular, it cleaves the N-terminal globular region of collagen I and II, leading to fibril formation. Mature human proADAMTS3 is a secreted, 1185 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 38-201), a peptidase M12B domain (aa 256-460), a disintegrin region (aa 470-550), four TSP type I sequences (aa 551-1015), and a PLAC domain (aa 1015-1054). There are two potential splice variants. One shows a 38 aa substitution for aa 169-1205, while another shows an alternative start site at Met171. Over aa 19-712, human ADAMTS3 shares 91% aa identity with mouse ADAMTS3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ADAMTS3
Uniprot