![Western Blot: AGER Antibody (JF0975) [NBP2-67095] - Analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Western-Blot-NBP2-67095-img0005.jpg "Western Blot: AGER Antibody (JF0975) [NBP2-67095] - Analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | JF0975 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human AGER aa 350-390. (SwissProt: Q15109 Human; SwissProt: Q62151 Mouse; SwissProt: Q63495 Rat) |
| Localization | Cell membrane, Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | AGER |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
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![Immunohistochemistry EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/EN-RAGE_AF1052_Immunohistochemistry_6671.jpg)
![Western Blot EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/ENRAGE_AF1052_Western_Blot_19098.jpg)
![Simple Western EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/af1052_human-en-rage-affinity-purified-polyclonal-ab-simple-western-2672021152820.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 12/07/2021 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AGER |
![Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Immunocytochemistry-Immunofluorescence-NBP2-67095-img0003.jpg "Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Mouse lung cancer tissue stained with AGER antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Immunohistochemistry-Paraffin-NBP2-67095-img0006.jpg "Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Mouse lung cancer tissue stained with AGER antibody. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095] - Analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Flow-Cytometry-NBP2-67095-img0001.jpg "Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095] - Analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Immunocytochemistry-Immunofluorescence-NBP2-67095-img0002.jpg "Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/AGER-Antibody-JF0975-Immunohistochemistry-Paraffin-NBP2-67095-img0004.jpg "Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
