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Acetyl CoA synthetase Antibody (OTI3H4)

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Western Blot: Acetyl CoA synthetase Antibody (3H4) [NBP2-01269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Acetyl CoA synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Acetyl CoA synthetase.
Western Blot: Acetyl CoA synthetase Antibody (3H4) [NBP2-01269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Acetyl CoA synthetase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody (3H4) [NBP2-01269] Staining of paraffin-embedded Human pancreas tissue using anti-Acetyl CoA synthetase mouse monoclonal antibody.
Flow Cytometry: Acetyl CoA synthetase Antibody (3H4) [NBP2-01269] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Acetyl CoA ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI3H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72039AF350
Alexa Fluor 405
NBP2-72039AF405
Alexa Fluor 488
NBP2-72039AF488
Alexa Fluor 532
NBP2-72039AF532
Alexa Fluor 594
NBP2-72039AF594
Alexa Fluor 647
NBP2-72039AF647
Alexa Fluor 700
NBP2-72039AF700
Alexa Fluor 750
NBP2-72039AF750
Allophycocyanin
NBP2-72039APC
Allophycocyanin/Cy7
NBP2-72039APCCY7
Biotin
NBP2-72039B
CoraFluor 1
NBP2-72039CL1
DyLight 350
NBP2-72039UV
DyLight 405
NBP2-72039V
DyLight 488
NBP2-72039G
DyLight 550
NBP2-72039R
DyLight 594
NBP2-72039DL594
DyLight 650
NBP2-72039C
DyLight 680
NBP2-72039FR
DyLight 755
NBP2-72039IR
FITC
NBP2-72039F
HRP
NBP2-72039H
Janelia Fluor 525
NBP2-72039JF525
Janelia Fluor 549
NBP2-72039JF549
Janelia Fluor 646
NBP2-72039JF646
Janelia Fluor 669
NBP2-72039JF669
PE
NBP2-72039PE
PE/Atto594
NBP2-72039PEATT594
PE/Cy5.5
NBP2-72039PECY55
PE/Cy7
NBP2-72039PECY7
PerCP
NBP2-72039PCP
mFluor Violet 450 SE
NBP2-72039MFV450
mFluor Violet 500 SE
NBP2-72039MFV500
mFluor Violet 610 SE
NBP2-72039MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72039
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Acetyl CoA synthetase Antibody (OTI3H4) Summary

Immunogen
Full length human recombinant protein of human ACSS2(NP_061147) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACSS2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
78.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01269.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Acetyl CoA synthetase Antibody (OTI3H4)

  • ACAS2
  • AceCS
  • acetate thiokinase
  • acetate-CoA ligase
  • Acetate--CoA ligase
  • Acetyl-CoA synthetase
  • acetyl-Coenzyme A synthetase 2 (ADP forming)
  • acetyl-coenzyme A synthetase, cytoplasmic
  • ACSA
  • ACSACECS
  • Acyl-activating enzyme
  • acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
  • cytoplasmic acetyl-coenzyme A synthetase
  • dJ1161H23.1
  • DKFZp762G026
  • EC 6.2.1

Background

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Acetyl CoA
synthetase Antibody (
NBP2-01269
Acetyl CoA synthetase Antibody (OTI3H4)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC

Publications for Acetyl CoA synthetase Antibody (NBP2-01269)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-01269 Applications Species
Thomas SP, Denu JM. Short-chain fatty acids activate acetyltransferase p300 eLife 2021-10-22 [PMID: 34677127]

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Bioinformatics

Gene Symbol ACSS2
Entrez