modENCODE-Antibodies reagents: Novus Biologicals

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67 Results for "modENCODE-Antibodies"

NB100-338

NDC80 Antibody (9G3.23)

(5 Reviews)

(48 Publications)

0.1 ml

NB100-338

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .

<b>Orthogonal Strategies Validation. </b>Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu, Mu, Ha, Mar

Species Glossary

Reactivity: Hu, Mu, Ha, Mar

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-87396

SUN1 Antibody

(14 Publications)

0.1 ml

NBP1-87396

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87396 (A) shows similar protein distribution across tissues to independent antibody NBP1-87395 (B).

Western Blot: SUN1 Antibody [NBP1-87396] - Western blot analysis of protein expression levels in control and irradiated MCF7 and U2OS cells. Blot cuts showing signals of intact proteins. Note, reduction of SUN2 protein and near absence of H3K9me3 epigenetic modification in senescent cells (7 days post irradiation). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/4/999), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of SUN1 a& nesprin-1 mislocalization in MCF7 and U2OS cells 24 h PI with 8 Gy of gamma-rays. 1a-Central slices through nucleus of MCF7 control show SUN1 & nesprin-1 in whole nucleus. 2-Convoluted nucleus of U2OS. 2a-Slice showing interrupted LA/C. 3-Convoluted nucleus of MCF7 containing 2 large MN, several blisters, and small MN bordered by LAC and filled by SUN1. 5-Tweens of MCF7 daughter nuclei connected by a thin anaphase bridge have fragmented SUN1 in the NE (arrows). 6-U2OS nucleus with amplified SUN1 and a clump (arrow) containing SUN1 and nesprin-1. Size bar indicates 5um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/4/999), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of A431 cells at 4ug/ml against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.

Simple Western: SUN1 Antibody [NBP1-87396] - Simple Western lane view shows a specific band for SUN1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SUN1 Antibody [NBP1-87396] - Electropherogram image(s) of corresponding Simple Western lane view. SUN1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of nuclear morphology defects in (A) MCF7 & (B) U2OS cell lines 24 h PI with the dose of 8 Gy of gamma -rays. (A) 1—An anaphase bridge linking the separating daughter nuclei, SUN1 (red), nesprin1 (green), 2—Convoluted nucleus with blisters (arrows), LA/C (green), emerin (red). 3—Fragmented nucleus represented by 3 large & numerous small micronuclei. SUN2 (red), nesprin-1 (green). (B) 1— A giant nucleus with small chromatin fragments & micronuclei (MN, arrows), SUN1 (red), nesprin-1 (green). 2—Nucleus with micronuclei, emerin (red), lamin A/C (green), arrows indicate MN containing emerin & heterochromatin (HC), 3—Convoluted nucleus with blisters (arrows), emerin (red), LA/C (green). 4—Fragmented nucleus, emerin (red), LA/C (green). 5—Honeycombed nucleus, emerin (red), LA/C (green), thick arrow shows interrupted NE enabling chromatin to leak out of the nucleus, reinforced layer of LA/C (green) & emerin (red), (thin arrows). Size bar indicates µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32316379), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-499

Rad51 Antibody (13E4)

(9 Publications)

100 ul

NB100-499

Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.

Western Blot: Rad51 Antibody (13E4) [NB100-499] - Sample (50ug of whole cell lysate) A: mouse testis 10% SDS PAGE diluted at 1:500

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-91848

DPY30 Antibody

(1 Review)

0.1 ml

NBP1-91848

Western Blot: DPY30 Antibody [NBP1-91848] - 40 ug total protein per lane, separated by 12% SDS-PAGE and blotted onto a PVDF membrane. DPY30 antibody at 1:200, GAPDH antibody at 1:3000. Secondary antibody at 1:5000. Detection by ECL. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: DPY30 Antibody [NBP1-91848] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.

Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human pancreas shows nuclear positivity.

Immunohistochemistry-Paraffin: DPY30 Antibody [NBP1-91848] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-75640

Rad51 Antibody (JM54-26)

100 ul

NBP2-75640

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.

Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.

Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-87395

SUN1 Antibody

(1 Publication)

0.1 ml

NBP1-87395

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87395 (A) shows similar protein distribution across tissues to independent antibody NBP1-87396 (B).

<b>Independent Antibodies Validation. </b>Western Blot: SUN1 Antibody [NBP1-87395] - Analysis using Anti-SUN1 antibody NBP1-87395 (A) shows similar pattern to independent antibody NBP1-87396 (B).

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87395] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & vesicles.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-16632

CHD3 Antibody (8Z6L6)

100 ul

NBP3-16632

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunocytochemistry/Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunofluorescence analysis of NIH-3T3 cells using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded mouse brain using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of various cell lines, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Rat brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded rat spleen using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded human appendix using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-20058

Rad51 Antibody

(1 Publication)

0.1 ml

NBP2-20058

Western Blot: Rad51 Antibody [NBP2-20058] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody.

<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human ovarian carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Rad51 Antibody [NBP2-20058] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Rad51 Antibody [NBP2-20058] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human breast carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: Rad51 Antibody [NBP2-20058] - Jurkat whole cell extract A. 40 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Rad51 protein by 4 ug Rad51 antibody 5 % SDS-PAGE The immunoprecipitated Rad51 protein was detected by Rad51 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent].

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-25702

SUN1 Antibody (HL1947) - Azide and BSA Free

100 ul

NBP3-25702

Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

<b>Orthogonal Strategies Validation. </b>Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

<b>Orthogonal Strategies Validation. </b>Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-13726

NDC80 [p Ser55] Antibody

100 ul

NBP3-13726

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDC80 [p Ser55] Antibody [NBP3-13726] - Immunofluorescent staining of NDC80 phospho Ser55 in cells treated with NDC80 siRNA (bottom) or control siRNA (top)

<b>Biological Strategies Validation. </b>Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 (phospho Ser 55) antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)

Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Mar

Species Glossary

Reactivity: Hu, Mu, Rt, Mar

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-03325

ZIM2 Antibody (OTI7G1)

0.1 ml

NBP2-03325

Western Blot: ZIM2 Antibody (OTI7G1) [NBP2-03325] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZIM2.

Immunocytochemistry/Immunofluorescence: ZIM2 Antibody (OTI7G1) [NBP2-03325] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ZIM2.

Immunohistochemistry-Paraffin: ZIM2 Antibody (OTI7G1) [NBP2-03325] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZIM2 mouse monoclonal antibody.

Flow Cytometry: ZIM2 Antibody (OTI7G1) [NBP2-03325] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZIM2 antibody, and then analyzed by flow cytometry.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74929AF350
Alexa Fluor 405	NBP2-74929AF405
Alexa Fluor 488	NBP2-74929AF488
Alexa Fluor 532	NBP2-74929AF532
Alexa Fluor 594	NBP2-74929AF594
Alexa Fluor 647	NBP2-74929AF647
Alexa Fluor 700	NBP2-74929AF700
Alexa Fluor 750	NBP2-74929AF750
Allophycocyanin	NBP2-74929APC
Biotin	NBP2-74929B
CoraFluor 1	NBP2-74929CL1
DyLight 350	NBP2-74929UV
DyLight 405	NBP2-74929V
DyLight 488	NBP2-74929G
DyLight 550	NBP2-74929R
DyLight 594	NBP2-74929DL594
DyLight 650	NBP2-74929C
DyLight 680	NBP2-74929FR
DyLight 755	NBP2-74929IR
FITC	NBP2-74929F
HRP	NBP2-74929H
Janelia Fluor 525	NBP2-74929JF525
Janelia Fluor 549	NBP2-74929JF549
Janelia Fluor 585	NBP2-74929JF585
Janelia Fluor 635	NBP2-74929JF635
Janelia Fluor 646	NBP2-74929JF646
Janelia Fluor 669	NBP2-74929JF669
PE	NBP2-74929PE
PerCP	NBP2-74929PCP
mFluor Violet 450 SE	NBP2-74929MFV450
mFluor Violet 500 SE	NBP2-74929MFV500
mFluor Violet 610 SE	NBP2-74929MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74929

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NBP3-06445

NASP Antibody

50 ul

NBP3-06445

Western Blot: NASP Antibody [NBP3-06445] - NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :180 kDa.

Immunocytochemistry/Immunofluorescence: NASP Antibody [NBP3-06445] - Immunofluorescence staining of NASP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NASP polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Immunohistochemistry-Paraffin: NASP Antibody [NBP3-06445] - Immunochemical staining of human NASP in human rectum with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

Western Blot: NASP Antibody [NBP3-06445] - Anti-NASP rabbit polyclonal antibody at 1:2000 dilution. Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:180 kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-06445AF350
Alexa Fluor 405	NBP3-06445AF405
Alexa Fluor 488	NBP3-06445AF488
Alexa Fluor 532	NBP3-06445AF532
Alexa Fluor 594	NBP3-06445AF594
Alexa Fluor 647	NBP3-06445AF647
Alexa Fluor 700	NBP3-06445AF700
Alexa Fluor 750	NBP3-06445AF750
Allophycocyanin	NBP3-06445APC
Biotin	NBP3-06445B
CoraFluor 1	NBP3-06445CL1
DyLight 350	NBP3-06445UV
DyLight 405	NBP3-06445V
DyLight 488	NBP3-06445G
DyLight 550	NBP3-06445R
DyLight 594	NBP3-06445DL594
DyLight 650	NBP3-06445C
DyLight 680	NBP3-06445FR
DyLight 755	NBP3-06445IR
FITC	NBP3-06445F
HRP	NBP3-06445H
Janelia Fluor 525	NBP3-06445JF525
Janelia Fluor 549	NBP3-06445JF549
Janelia Fluor 585	NBP3-06445JF585
Janelia Fluor 635	NBP3-06445JF635
Janelia Fluor 646	NBP3-06445JF646
Janelia Fluor 669	NBP3-06445JF669
PE	NBP3-06445PE
PerCP	NBP3-06445PCP
mFluor Violet 450 SE	NBP3-06445MFV450
mFluor Violet 500 SE	NBP3-06445MFV500
mFluor Violet 610 SE	NBP3-06445MFV610

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