![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0007.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | JM54-26 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Rad51 aa 1-50 / 339. (SwissProt: Q06609 Human; SwissProt: Q08297 Mouse; SwissProt: B5DF04 Rat) |
| Localization | Chromosome, Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RAD51 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad51 Antibody (NBP2-75640)Find related products by research area.
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
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RAD51: The cell's 'Mr. Fix-it' RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo... Read full blog post. |
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NUP153 & 53BP1: A Novel DNA Repair Pathway Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De... Read full blog post. |
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Determining DMC1's role in Homologus Recombination The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb... Read full blog post. |
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Rad51 Antibody Reveals a Canine Model for Human Breast Cancer Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb... Read full blog post. |
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Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con... Read full blog post. |
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| Gene Symbol | RAD51 |
![Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Flow-Cytometry-NBP2-75640-img0001.jpg "Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0005.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0006.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
![Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-western-blot-2862024111350..jpg "Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111534..jpg "Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111720..jpg "Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024111850..jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-286202411203..jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024112111..jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")