![Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249362771.jpg "Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1947 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human SUN1 |
| Predicted Species | Chimpanzee (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SUN1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 90 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SUN1 Antibody (NBP3-25702)Find related products by research area.
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| Gene Symbol | SUN1 |
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249393549.jpg "Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-212024934271.jpg "Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249435513.jpg "Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249432854.jpg "Immunohistochemistry-Paraffin: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249393526.jpg "Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-212024940873.jpg "Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")