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XCL1/Lymphotactin Antibody [Unconjugated]

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Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dosdependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human XCL1/Lymphotactin (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695). The ND50 is typically 10-30 µg/mL.
Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dosdependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF695
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF695-SP
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XCL1/Lymphotactin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human XCL1/Lymphotactin
Val22-Gly114
Accession # P47992
Specificity
Ddetects human XCL1/Lymphotactin in direct ELISAs and Western blots. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XCL1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 3.00-30.0 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF695 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody [Unconjugated]

  • ATAC
  • ATACSmall-inducible cytokine C1
  • C motif chemokine 1
  • chemokine (C motif) ligand 1
  • Cytokine SCM-1
  • LPTNSCM-1-alpha
  • LTNXC chemokine ligand 1
  • Lymphotactin
  • lymphotaxin
  • SCM-1 alpha
  • SCM-1
  • SCM1A
  • SCM-1a
  • SCYC1SCM1
  • single cysteine motif 1a
  • small inducible cytokine subfamily C, member 1 (lymphotactin)
  • XCL1

Background

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4+ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

  1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
  2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF695
XCL1/Lymphotactin Antibody [Unconjugated]
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Publications for XCL1/Lymphotactin Antibody (AF695)(2)

We have publications tested in 2 confirmed species: Human, Virus - HIV-1.

We have publications tested in 3 applications: Capture Assay, Co-Immunoprecipitation, Flow Cytometry.


Filter By Application
Capture Assay
(1)
Co-Immunoprecipitation
(1)
Flow Cytometry
(1)
All Applications
Filter By Species
Human
(2)
Virus - HIV-1
(1)
All Species

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol XCL1
Uniprot