![Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Xp, ZeSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary. |
| Localization | Cytoplasm; Membrane; Peripheral membrane protein |
| Predicted Species | Rat (100%), Chicken (98%), Xenopus (97%), Bovine (100%), Zebrafish (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VPS35 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 92 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-20876 | Applications | Species |
|---|---|---|
| Yin J, Liu X, He Q et al. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic 2016-09-26 [PMID: 27717139] |
Secondary Antibodies |
Isotype Controls |
Research Areas for VPS35 Antibody (NBP2-20876)Find related products by research area.
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![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-20876-img0003.jpg "Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min")
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0001.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0002.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunoprecipitation-NBP2-20876-img0006.jpg "Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")