VPS35 Antibodies
We offer VPS35 Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each VPS35 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our VPS35 Antibodies can be used in a variety of model species: Chinese Hamster, Human, Mouse, Rat. Use the list below to choose the VPS35 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our VPS35 polyclonal antibodies and browse our VPS35 monoclonal antibody catalog.
Alternate Names for VPS35 Antibodies
anti-VPS35 antibody, anti-DKFZp434P1672 antibody, anti-FLJ10752 antibody, anti-FLJ13588 antibody, anti-FLJ20388 antibody, anti-hVPS35 antibody, anti-Maternal-embryonic 3 antibody, anti-MEM3DKFZp434E1211 antibody, anti-vacuolar protein sorting 35 (yeast homolog) antibody, anti-vacuolar protein sorting 35 (yeast) antibody, anti-vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, anti-vacuolar protein sorting-associated protein 35 antibody, anti-Vesicle protein sorting 35 antibody
11 Results for "vps35" in Primary Antibodies
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0009.jpg "Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0011.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0005.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 for 1 hr at room temp. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0006.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0007.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at 1:2000 overnight in 1% BSA at 4 degrees. ICC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-NB100-1397-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0004.jpg)
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0002.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.")
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0001.jpg)
![ELISA: VPS35 Antibody (2D3) [H00055737-M02] - Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-ELISA-H00055737-M02-img0003.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (NBP3-13680) diluted at 1:500.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunocytochemistry-Immunofluorescence-NBP3-13680-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunohistochemistry-Paraffin-NBP3-13680-img0005.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0001.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0002.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0003.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202419593560.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202420131291.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242091446.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242011446.jpg)
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0013.jpg "Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.")
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0012.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. Clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0010.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: MEF, A549 cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (SMC-602) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0011.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP1-31069-img0017.jpg "Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.")
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0004.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0005.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0016.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VPS35 stained by VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-91020242091483.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-91020242041261.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-1610202419414583.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0012.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: Intestinal Cancer. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunohistochemistry-NBP2-78827-img0013.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 11H10 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0007.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of MEF lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0010.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-20876-img0003.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0001.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunoprecipitation-NBP2-20876-img0006.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-13517-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13517-img0008.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-13517-img0007.jpg)
![Western Blot: VPS35 Antibody (S03-6E1) [NBP3-19926] - Western blot detection of VPS35 in HepG2, U2OS, U251 using NBP3-19926 (1:1000 diluted)](http://images.novusbio.com/fullsize/VPS35-Antibody-S03-6E1-Western-Blot-NBP3-19926-img0002.jpg "Western Blot: VPS35 Antibody (S03-6E1) [NBP3-19926] - Western blot detection of VPS35 in HepG2, U2OS, U251 using NBP3-19926 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: VPS35 Antibody (S03-6E1) [NBP3-19926] - Immunohistochemistry of VPS35 in paraffin-embedded Human lung cancer tissue using NBP3-19926 at dilution 1/50](http://images.novusbio.com/fullsize/VPS35-Antibody-S03-6E1-Immunohistochemistry-Paraffin-NBP3-19926-img0001.jpg)
![Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803] - Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (NBP3-16803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/VPS35-Antibody-8D3L3-Western-Blot-NBP3-16803-img0001.jpg "Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803] - Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (NBP3-16803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")