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VPS29 Antibody

Images

 
Western Blot: VPS29 Antibody [NBP3-12366] - Rat lung extract. 1:500 antibody dilution in DiluObuffer. Apparent MW is 26 KDa.
Immunohistochemistry-Paraffin: VPS29 Antibody [NBP3-12366] - Mouse Kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: VPS29 Antibody [NBP3-12366] - Mouse Spleen. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VPS29 Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 133-183 on rat Vacuolar Protein Sorting-Associated Protein 29.
Clonality
Polyclonal
Host
Rabbit
Gene
VPS29
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for VPS29 Antibody

  • DC15
  • DC7
  • DKFZp564F0223
  • EC 3.1.3.3
  • FLJ20492
  • hVPS29
  • PEP11 homolog
  • PEP11
  • retromer protein
  • vacuolar protein sorting 29 (yeast homolog)
  • vacuolar protein sorting 29 (yeast)
  • vacuolar protein sorting 29 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 29
  • vacuolar sorting protein VPS29/PEP11
  • Vesicle protein sorting 29
  • x 007 protein

Background

VPS29 is an essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS29