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![Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0009.jpg "Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0011.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0005.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 for 1 hr at room temp. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0006.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0007.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NB100-1397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at 1:2000 overnight in 1% BSA at 4 degrees. ICC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-NB100-1397-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0004.jpg)
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0002.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.")
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-Western-Blot-H00055737-M02-img0001.jpg)
![ELISA: VPS35 Antibody (2D3) [H00055737-M02] - Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-2D3-ELISA-H00055737-M02-img0003.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (NBP3-13680) diluted at 1:500.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunocytochemistry-Immunofluorescence-NBP3-13680-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunohistochemistry-Paraffin-NBP3-13680-img0005.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0001.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0002.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0003.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202419593560.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202420131291.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242091446.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242011446.jpg)
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0013.jpg "Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.")
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0012.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. Clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0010.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: MEF, A549 cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (SMC-602) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0011.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP1-31069-img0017.jpg "Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.")
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0004.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0005.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP1-31069-img0016.jpg)
![Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VPS35 stained by VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-91020242091483.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (NBP1-31069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-91020242041261.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31069_rabbit-polyclonal-vps35-antibody-1610202419414583.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0012.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: Intestinal Cancer. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunohistochemistry-NBP2-78827-img0013.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 11H10 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0007.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of MEF lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0010.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-20876-img0003.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0001.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-20876-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunoprecipitation-NBP2-20876-img0006.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-13517-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13517-img0008.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/VPS35-Antibody-Western-Blot-NBP2-13517-img0007.jpg)
![Western Blot: VPS35 Antibody (S03-6E1) [NBP3-19926] - Western blot detection of VPS35 in HepG2, U2OS, U251 using NBP3-19926 (1:1000 diluted)](http://images.novusbio.com/fullsize/VPS35-Antibody-S03-6E1-Western-Blot-NBP3-19926-img0002.jpg "Western Blot: VPS35 Antibody (S03-6E1) [NBP3-19926] - Western blot detection of VPS35 in HepG2, U2OS, U251 using NBP3-19926 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: VPS35 Antibody (S03-6E1) [NBP3-19926] - Immunohistochemistry of VPS35 in paraffin-embedded Human lung cancer tissue using NBP3-19926 at dilution 1/50](http://images.novusbio.com/fullsize/VPS35-Antibody-S03-6E1-Immunohistochemistry-Paraffin-NBP3-19926-img0001.jpg)
![SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-VPS35-Protein-SDS-Page-H00055737-P01-img0001.jpg "SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0001.jpg "ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve")
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0002.jpg)
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0003.jpg)