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VPS35 Antibody

Images

 
Genetic Strategies: Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.
Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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VPS35 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: SCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS35
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
VPS35 Protein (NBP2-13517PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VPS35 Antibody

  • DKFZp434P1672
  • FLJ10752
  • FLJ13588
  • FLJ20388
  • hVPS35
  • Maternal-embryonic 3
  • MEM3DKFZp434E1211
  • vacuolar protein sorting 35 (yeast homolog)
  • vacuolar protein sorting 35 (yeast)
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 35
  • Vesicle protein sorting 35

Background

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS35 Antibody (NBP2-13517) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS35