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Von Hippel Lindau Antibody (1066402) [Unconjugated]

 
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Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cells, loaded at 0.2 mg/mL. A specific band was detected for VHL at approximately 23 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cells, loaded at 0.2 mg/mL. A specific band was detected for VHL at approximately 23 kDa (as indicated) using 20 µg/mL of Mouse ...read more
Western blot shows lysates of K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
1066402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB11435-SP
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Von Hippel Lindau Antibody (1066402) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337
Specificity
Detects human VHL in direct ELISA.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Von Hippel Lindau Antibody (1066402) [Unconjugated]

  • elongin binding protein
  • HRCA1
  • Protein G7
  • pVHL
  • RCA1
  • VHL
  • VHL1
  • von Hippel-Lindau disease tumor suppressor
  • von Hippel-Lindau syndrome
  • von Hippel-Lindau tumor suppressor

Background

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha , beta 2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  
  1. Kamura T., et al. (1998) Genes & Dev.  doi: 10.1101/gad.12.24.3872
  2. Okumura F., et al. (2012) Front. Oncol.  doi: 10.3389/fonc.2012.00010
  3. Xie L., et al. (2009) Sci. Signaling  doi: 10.1126/scisignal.2000444
  4. Zhang J., et al. (2018) Science  doi: 10.1126/science.aap8411

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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