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Vitronectin Antibody

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Immunocytochemistry/Immunofluorescence: Vitronectin Antibody [NBP2-38901] - Staining of human cell line CACO-2 shows localization to endoplasmic reticulum & vesicles.
Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody [NBP2-38901] - Staining of human cell line CACO-2 shows localization to endoplasmic reticulum & vesicles.
Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-38901] - Staining of human kidney shows distinct positivity in plasma.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Vitronectin Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VTN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Vitronectin Recombinant Protein Antigen (NBP2-38901PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Vitronectin Antibody

  • Complement S-protein
  • epibolin
  • Serum Spreading Factor
  • Serum-spreading factor
  • Somatomedin B
  • S-protein
  • V75
  • vitronectin (serum spreading factor, somatomedin B, complement S-protein)
  • Vitronectin
  • VN
  • VNT
  • VTN

Background

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VTN
Entrez
Uniprot