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Villin 1 Antibody

Images

 
Independent Antibodies: Western Blot: Villin 1 Antibody [NBP1-85335] - Analysis using Anti-VIL1 antibody NBP1-85335 (A) shows similar pattern to independent antibody NBP1-85336 (B).
Immunocytochemistry/ Immunofluorescence: Villin 1 Antibody [NBP1-85335] - Staining of human cell line CACO-2 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon.
Independent Antibodies: Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85335 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human liver.
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human gallbladder.
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human kidney.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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Villin 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Villin 1 Protein (NBP1-85335PEP)
Publications
Read Publications using
NBP1-85335 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Villin 1 Antibody

  • D2S1471
  • VIL
  • villin 1
  • villin-1

Background

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Villin 1 Antibody (NBP1-85335)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry.


Filter By Application
IF/IHC
(1)
Immunocytochemistry/ Immunofluorescence
(1)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(2)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Villin 1 Antibody (NBP1-85335) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VIL1
Entrez
Uniprot