| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52585-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52584-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54515-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-50081-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79943 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08935 | |||
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0008.jpg "Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.")
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0004.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0007.jpg)
![Flow Cytometry: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of HeLa cells with Villin1 antibody at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Flow-Cytometry-NBP2-75707-img0001.jpg)
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Caco-2 cell line lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0006.jpg)
![Western Blot: Villin 1 Antibody [NBP1-85335] - Analysis using Anti-VIL1 antibody NBP1-85335 (A) shows similar pattern to independent antibody NBP1-85336 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Western-Blot-NBP1-85335-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Villin 1 Antibody [NBP1-85335] - Analysis using Anti-VIL1 antibody NBP1-85335 (A) shows similar pattern to independent antibody NBP1-85336 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-85335] - Staining of human cell line CACO-2 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85335-img0002.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85335 (A) shows similar protein distribution across tissues to independent antibody NBP1-85336 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human liver.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0006.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85335] - Staining of human kidney.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85335-img0008.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85336 (A) shows similar protein distribution across tissues to independent antibody NBP1-85335 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody NBP1-85336 (A) shows similar protein distribution across tissues to independent antibody NBP1-85335 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Villin 1 Antibody [NBP1-85336] - Analysis using Anti-VIL1 antibody NBP1-85336 (A) shows similar pattern to independent antibody NBP1-85335 (B).](http://images.novusbio.com/fullsize/Villin-1-Antibody-Western-Blot-NBP1-85336-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-85336] - Staining of human cell line CACO-2 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85336-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human colon.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0013.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0008.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human pancreas shows weak to moderate positivity in luminal membranes in exocrine glandular cells.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0009.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0010.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human liver.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0011.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0012.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-85336] - Staining of human kidney using Anti-VIL1 antibody NBP1-85336.](http://images.novusbio.com/fullsize/Villin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85336-img0014.jpg)
![Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mouse mAb against SW620 cell lysate.](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Western-Blot-NBP2-52585-img0007.jpg "Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mouse mAb against SW620 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Immunocytochemistry-Immunofluorescence-NBP2-52585-img0006.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of paraffin-embedded cervical cancer tissues using VIL1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Immunohistochemistry-Paraffin-NBP2-52585-img0003.jpg)
![Flow Cytometry: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of SW620 cells using VIL1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Flow-Cytometry-NBP2-52585-img0001.jpg)
![Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Western-Blot-NBP2-52585-img0005.jpg)
![Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-Western-Blot-NBP2-52585-img0004.jpg)
![ELISA: Villin 1 Antibody (3E5G11) [NBP2-52585] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Villin-1-Antibody-3E5G11-ELISA-NBP2-52585-img0008.jpg)
![Western Blot: Villin 1 Antibody (5E3B2) [NBP2-52584] - Analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)](http://images.novusbio.com/fullsize/Villin-1-Antibody-5E3B2-Western-Blot-NBP2-52584-img0003.jpg "Western Blot: Villin 1 Antibody (5E3B2) [NBP2-52584] - Analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (5E3B2) [NBP2-52584] - Analysis of paraffin-embedded stomach cancer tissues using VIL1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Villin-1-Antibody-5E3B2-Immunohistochemistry-Paraffin-NBP2-52584-img0002.jpg)
![Western Blot: Villin 1 Antibody (5E3B2) [NBP2-52584] - Analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Villin-1-Antibody-5E3B2-Western-Blot-NBP2-52584-img0004.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (5E3B2) [NBP2-52584] - Analysis of paraffin-embedded muscle tissues using VIL1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Villin-1-Antibody-5E3B2-Immunohistochemistry-Paraffin-NBP2-52584-img0001.jpg)
![ELISA: Villin 1 Antibody (5E3B2) [NBP2-52584] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Villin-1-Antibody-5E3B2-ELISA-NBP2-52584-img0005.jpg)
![Western Blot: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1325-Western-Blot-NBP2-53201-img0003.jpg "Western Blot: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1325).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1325-Immunohistochemistry-Paraffin-NBP2-53201-img0002.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1325) [NBP2-53201] - Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1325-Immunohistochemistry-Paraffin-NBP2-53201-img0001.jpg)
![Western Blot: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Western-Blot-NBP2-53195-img0003.jpg "Western Blot: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1314).")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Immunohistochemistry-Paraffin-NBP2-53195-img0002.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/1314) [NBP2-53195] - Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1314).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-1314-Immunohistochemistry-Paraffin-NBP2-53195-img0001.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) [NBP2-79809] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Villin 1 Antibody (rVIL1/1325).](http://images.novusbio.com/fullsize/Villin-1-Antibody-rVIL1-1325-Immunohistochemistry-Paraffin-NBP2-79809-img0002.jpg "Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) [NBP2-79809] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Villin 1 Antibody (rVIL1/1325).")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (rVIL1/1325) [NBP2-79809] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Villin 1 Antibody (rVIL1/1325).](http://images.novusbio.com/fullsize/Villin-1-Antibody-rVIL1-1325-Immunohistochemistry-Paraffin-NBP2-79809-img0001.jpg)
![Protein Array: Villin 1 Antibody (rVIL1/1325) [NBP2-79809] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Villin 1 Antibody (rVIL1/1325) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Villin-1-Antibody-rVIL1-1325-Protein-Array-NBP2-79809-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/4107R) [NBP3-07190] - Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin 1 Recombinant Rabbit Monoclonal Antibody (VIL1/4107R).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-4107R-Immunohistochemistry-Paraffin-NBP3-07190-img0002.jpg "Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/4107R) [NBP3-07190] - Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin 1 Recombinant Rabbit Monoclonal Antibody (VIL1/4107R).")
![Immunohistochemistry-Paraffin: Villin 1 Antibody (VIL1/4107R) [NBP3-07190] - Formalin-fixed, paraffin-embedded human colon stained with Villin 1 Recombinant Rabbit Monoclonal Antibody (VIL1/4107R).](http://images.novusbio.com/fullsize/Villin-1-Antibody-VIL1-4107R-Immunohistochemistry-Paraffin-NBP3-07190-img0001.jpg)
![SDS-Page: Recombinant Human Villin 1 Protein [H00007429-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007429-P01-1.jpg "SDS-Page: Recombinant Human Villin 1 Protein [H00007429-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Villin 1 Antibody (A2) [NBP3-33091] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33091_rabbit-villin-1-mab-a2-28820241419152.jpg "Western Blot: Villin 1 Antibody (A2) [NBP3-33091] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Villin 1 Antibody (A2) [NBP3-33091] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33091) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33091_rabbit-villin-1-mab-a2-29820241420928.jpg)
![Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-288202414191528.jpg "Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Villin 1 Antibody (C4) [NBP3-33090] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33090) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-29820241416187.jpg)